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Table 3 Real Time PCR of potential proteins from heart sample

From: Identification of troponin I and actin, alpha cardiac muscle 1 as potential biomarkers for hearts of electrically stimulated chickens

Spot ID

Protein Name

Gene Name

Ct Value Sample

Ct Value Control

Ct Value Difference

A value

Fold Increment

b

ACTC1

ACTC1

17.0 ± 0.33

17.4 ± 0.35

0.38

-3.258

142.27

d

Troponin I

Troponin I

13.5 ± 0.02

13.7 ± 0.03

0.22

-3.374

45.43

  1. The genes are identified as alpha cardiac muscle actin 1 (ACTC1) and troponin I (TNNI3). Values are the means of triplicate experiments. "A" value for fold increment was obtained from the equation by Pfaffl, 2001.
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Proteome Science

ISSN: 1477-5956