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Table 4 List of primers designed for genes related to potential proteins identified from heart sample

From: Identification of troponin I and actin, alpha cardiac muscle 1 as potential biomarkers for hearts of electrically stimulated chickens

Target gene

Left primer

Right primer

Product size

ACTC1

5'-ctgatcgcatgcagaaagaa-3'

5'-acatttgctggaaggtggac-3'

138

Troponin I

5'-acgccaagagacaatccaag-3'

5'-gtttctctcctgccctctcc-3'

117

  1. The amplification process was optimized by Polymerase Chain Reaction (PCR) and the conditions used for Real Time PCR.