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Figure 1 | Proteome Science

Figure 1

From: Protein-based identification of quantitative trait loci associated with malignant transformation in two HER2+ cellular models of breast cancer

Figure 1

Representative 2-D proteomic profiles of cell lines (A) 184A1, (B) BT474 and (C) SKBR3. The first dimension was resolved on IPG strip 4-7, 7 cm. The second dimension is a 12% SDS-PAGE spanning molecular weight region 10-250 kDa, stained with coomassie blue and scanned using Typhoon 9400 scanner. (D) Quantitative image analysis using Ludesi REDFIN reveals the gel reproducibility and protein loading with no significant difference in the number of identified protein spots on the gel replicates across each cell line. Error bars represent S.E.M. Scatter plots of average normalized intensities are plotted on a logarithmic scale for matching protein spots showing the dynamic range of spot detection and correlation for the normal cell line 184A1 versus (E) BT474 and (F) SKBR3.

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Proteome Science

ISSN: 1477-5956