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Table 1 Proteins identified from vitreous of PDR

From: Characterisation of the vitreous proteome in proliferative diabetic retinopathy

Spot no.(a)

Protein name

Swiss-Prot Access

Biological process

Theoretical

Observed

Mascot Score(b)

Matched peptides

Ratio PDR: C(c)

    

MW (Da)

PI

MW (kDa)

PI

   

437

Ig alpha-1 chain C

P01876

immune response

37630

6.08

63

6.4

281

9

5.45

441

region

    

63

6.0

366

13

4.29

443

     

62

6.2

356

11

3.61

467

     

63

5.8

364

10

3.39

500

     

70

6.5

143

6

-13.51

486

Tubulin alpha-1B chain

P68363

microtubule-based movement

50119

4.94

67

6.9

100

10

2.36

520

Keratin, type II

P35908

keratinisation

65393

8.07

60

6.6

344

11

2.01

740

cytoskeletal 2

    

40

6.8

454

13

-7.87

838

epidermal

    

33

7.6

102

6

3.11

844

     

32

7.2

72

8

3.14

536

Complement factor I

P05156

immune response

65677

7.72

61

6.7

116

8

2.29

537

Beta-crystallin B1

P53674

visual perception

28006

8.59

62

6.8

101

7

2.89

603

     

60

5.2

120

7

-2.55

645

     

45

7.8

127

8

-5.77

573

Hemopexin

P02790

transport

51643

6.55

62

7.1

239

10

3.31

578

Cathepsin D

P07339

proteolysis

44524

6.1

48

5.5

592

19

-3.24

585

     

49

5.6

361

18

-2.85

580

Pigment

P36955

cell proliferation;

46313

5.97

50

5.7

119

6

-2.92

598

epithelium-derived

 

negative regulation of angiogenesis;

  

47

5.8

194

7

-2.96

599

factor (PEDF)

 

positive regulation of neurogenesis

  

60

6.2

339

11

-3.36

600

Gamma-enolase

P09104

glycolysis

47239

4.91

60

5.2

625

19

-25.63

639

Zinc-alpha-2-

P25311

fatty acid binding;

33851

6.45

45

5.2

251

12

3.52

654

glycoprotein

 

ribonuclease activity

  

43

5.4

150

13

2,99

667

     

43

5.3

394

17

4.65

689

Cytosolic acyl coenzyme A thioester hydrolase

O00154

hydrolysis

41769

8.85

40

7.6

134

7

-7.16

725

Ig heavy chain V-lll region BRO

P01766

immune response

13218

6.45

42

6.1

101

3

-6.14

726

N(G),N(G)-dimethylarginine dimethylaminohydrolase 1

O94760

arginine catabolic process;

signal transduction

31102

5.53

43

5.8

65

5

-6.74

736

Glyceraldehyde-3-phosphate dehydrogenase (GAPDH)

P04406

glycolysis

36030

8.57

37

8.3

308

11

-30.90

755

Clusterin

P10909

apoptosis;

52461

5.89

38

4.8

521

19

-3.43

763

  

immune response

  

40

4.5

314

12

-2.56

766

     

38

5.1

284

15

-2.21

797

     

34

5.5

114

2

-3.92

800

     

35

5.9

221

10

-2.24

765

Malate dehydrogenase

P40925

tricarboxylic acid cycle

36403

6.91

38

7.3

199

4

-4.74

794

Keratin, type I

P35527

intermediate filament organisation

62027

5.14

33

5.6

221

16

-2.20

1078

cytoskeletal 9

    

17

8.5

134

8

-14.34

838

Carbonic

P00918

dehydratase activity;

29228

6.87

32

8.2

223

8

3.11

854

anhydrase 2

    

32

7.6

136

4

3.54

855

  

zinc ion binding

  

33

7.7

390

13

3.13

845

Keratin, type II cytoskeletal 6A

P02538

structural constituent of cytoskeleton

60008

8.09

33

7.4

82

11

3.23

930

Ig lambda-1 chain C regions

P01842

immune response

11230

6.92

30

5.6

95

4

-2.61

942

Prostaglandin-H2

P41222

biosynthesis;

21015

7.66

28

4.9

91

4

-2.53

990

D-isomerase

 

transport

  

28

5.1

88

5

-3.32

974

Beta-crystallin S

P22914

structural constituent of eye lens

20993

6.44

28

6.8

147

4

-27.42

978

     

26

7.5

231

8

-24.32

1019

     

25

7.2

271

10

-57.43

982

Glutathione peroxidase 3

P22352

hydrogen peroxide catabolic process;

oxidation/reduction

25537

8.26

27

5.9

256

6

-2.49

984

Beta-crystallin B2

P43320

visual perception

23365

6.50

27

8.0

113

5

-15.21

1010

Phosphatidylethanolamine-binding protein 1

P30086

protease inhibitor

21044

7.01

28

8.3

437

12

-39.30

1015

Alpha-crystallin B chain

P02511

anti-apoptosis;

muscle contraction;

intracellular transport;

protein folding;

protein homooligomerisation;

response to heat

20146

6.76

26

7.8

283

9

-26.80

1049

Beta-crystallin A4

P53673

visual perception

22360

5.83

20

6.5

137

5

-5.70

1021

Beta-crystallin A3

P05813

visual perception

25134

5.81

25

6.8

483

12

-109.74

1022

     

23

7.9

428

11

-149.26

1023

     

24

7.5

351

12

-233.49

1024

Gamma-crystallin C

P07315

visual perception

20865

6.88

23

7.8

395

12

-147.83

  1. (a) In most cases, multiple spots correspond to the same protein.
  2. (b) Probability-based MOWSE score. Scores higher than 64 indicate the level of statistical significance at p < 0.05.
  3. (c) Abundance ratio between the different samples. A ratio > 2 or < -2 indicates statistical significance at p < 0.01. The fold-change of downregulation is shown as a negative number, and the fold-change of upregulation is shown as a positive number.
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