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Figure 1 | Proteome Science

Figure 1

From: Proteomic analysis of effluents from perfused human heart for transplantation: identification of potential biomarkers for ischemic heart damage

Figure 1

Chromatogram of the affinity removal of high-abundant proteins from the effluent. Injections of 75 ul of 4 × diluted effluent in buffer A were made on a Multiple Affinity Removal Column (4.6 × 50 mm) at a flow rate of 0.25 ml/min in buffer A. Flow-through fractions were collected from 1.5 - 4.5 min. The bound fraction was eluted with buffer B at a flow rate of 1.0 ml/min for 3.5 min.

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