Six proteins released significantly into the effluents during myocardial ischemia by quantitative analysis of the mean of the peak intensities (MPI). A. The MPI of L-lactate dehydrogenase B (LDHB). B. The MPI of glyceraldehyde-3-phosphate dehydrogenase (GAPDH). C. The MPI of glucose-6-phosphate isomerase (GPI). D. The MPI of phosphoglycerate mutase 2 (PGAM2). E. The MPI of gelsolin. F. The MPI of isoform 8 of titin. Among them, LDHB, GAPDH, GPI and PGAM2 are glycolytic enzymes. Gelsolin and isoform 8 of titin are targets of matrix metalloproteinase (MMP). Value are expressed as means ± SEM for each group. #p < 0.05 represents a significant difference in the ischemia for 30 min and ischemia for 60 min group compared with the ischemia for 0 min group; ^p < 0.05 represents a significant difference in the ischemia for 60 min group compared with the ischemia for 30 min group.