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Table 1 Most common proteins observed in 12 PPDj samples

From: Production and proteomic characterisation of purified protein derivative from Mycobacterium avium subsp. paratuberculosis

Accession

Locus_tag ‡

Locus_tag ¥

Gene

Mass (Da)

Product

PPDb

PPDa

20 W sec

34 W sec

P60533

MAPK_4266

MAP_4264

groES

10716.1

10 kDa chaperonin

+

+

+

+

Q73YF9

MAPK_1771

MAP_1997

acpM

12452.9

acyl carrier protein

+

+

+

+

Q8VU82

MAPK_2159

MAP_1609c

fbpB

34692.8

fibronectin-binding antigen 85 complex B

+

+

+

+

Q00488

MAPK_3842

MAP_3840

dnaK

66518.5

molecular chaperone DnaK

+

+

+

+

Q50320

MAPK_0241

MAP_3527

pepA

35709.1

serine protease

+

+

+

+

Q73UE0

MAPK_0340

MAP_3428c

 

23791.0

cutinase-related

 

+

+

+

Q73WQ3

MAPK_1161

MAP_2607c

fdxC

11792.1

ferredoxin

+

+

+

+

Q8GF25

MAPK_2115

MAP_1653

tpx

16684.8

thiol peroxidase

 

+

+

+

Q73YR6

MAPK_1879

MAP_1889c

wag31

28049.9

secreted antigen Wag31

+

+

 

+

Q73ZR1

MAPK_2228

MAP_1540

cfp17

16715.2

forkhead-domain protein

+

+

 

+

Q741F3

MAPK_2630

MAP_1138c

lprG

24720.8

lipoprotein

+

+

  

P42384

MAPK_3938

MAP_3936

groL2

56642.6

chaperonin GroEL2

+

+

+

+

Q741G8

MAPK_2646

MAP_1122

mIHF

12176.2

integration host factor MihF

+

+

+

+

A0QME6

MAPK_0122

MAP_3646

pckA

67659.5

phosphoenolpyruvate carboxykinase (GTP)

  

+

 

P60545

MAPK_4267

MAP_4265

groL1

55793.3

chaperonin GroEL

+

 

+

 

P61976

MAPK_1227

MAP_2541c

mdh

34599.2

malate dehydrogenase

  

+

+

Q73TQ9

MAPK_0109

MAP_3659

 

53750.8

long-chain-fatty-acid--CoA ligase

   

+

Q73VF3

MAPK_0707

MAP_3061c

fixA

27847.4

electron transfer flavoprotein (beta-subunit)

 

+

+

+

Q73VF4

MAPK_0708

MAP_3060c

fixB

32085.4

electron transfer flavoprotein (alpha-subunit)

+

 

+

 

Q73X57

MAPK_1315

MAP_2453c

atpA

59992.9

H(+)-transporting two-sector ATPase, alpha subunit

   

+

P61336

MAPK_0813

MAP_2955c

tsf

29065.9

elongation factor Tsf

+

 

+

+

Q73XM7

MAPK_1486

MAP_2282c

tig

50618.2

trigger factor

    

Q741U7

MAPK_2778

MAP_0990

eno

44841.2

phosphopyruvate hydratase

    

A0QLN4

MAPK_3950

MAP_3948c

 

17094.2

hypothetical protein

 

+

+

+

D5PC72

MAPK_4128

MAP_4126

rplL

13430.4

ribosomal protein L7/L12

+

+

+

+

Q73SD1

MAPK_4145

MAP_4143

tuf

43738.7

protein-synthesizing GTPase

+

+

+

+

Q73ZL3

MAPK_2179

MAP_1589c

ahpC

21641.4

peroxiredoxin subunit C

  

+

+

A0QCX8

MAPK_1317

MAP_2451c

atpD

53141.2

H(+)-transporting two-sector ATPase, beta subunit

    

Q73S77

MAPK_4201

MAP_4199

adk

19935.7

adenylate kinase

+

 

+

+

  1. The accession number for the protein with highest sequence coverage is given. Locus tag from the revised K10 genome sequence ‡ and original K10 genome sequence ¥, gene name, mass (Da) and protein name for the MAP K10 ortholog is provided. Proteins also identified in PPDb, PPDa, 20 week or 34 week culture filtrate are indicated by +