Proteomics analysis of differentially expressed proteins in chicken trachea and kidney after infection with the highly virulent and attenuated coronavirus infectious bronchitis virus in vivo

Table 1 Similar abundance changed proteins in tracheal from chicken infected with IBV ck/CH/LDL/97I P₅ and ck/CH/LDL/97I P₁₁₅

Match ID^a	Protein description	Accession no.^b	Protein score	Protein score CI%^c	Mw (Da)	pI	Average ratio and p-value
							P ₅ -infected/control								P ₁₁₅ -infected/control
							4 dpi		7 dpi		14 dpi		21 dpi		4 dpi		7 dpi		14 dpi		21 dpi
							ratio	p	ratio	p	ratio	p	ratio	p	ratio	p	ratio	p	ratio	p	ratio	p
4467	annexin A1 (ANXA1)	gi\|46195459	910	100	38761	7.05							0.89	0.435							0.49	0.001
2051	B-creatine kinase	gi\|211235	631	100	42525.5	5.78					1.44	0.025							1.77	0.006
1926, 1736, 1817, 4581	cartilage matrix protein precursor	gi\|71896317	314	100	54798.1	6.63	2.71	0.006			1.24	0.427	1.94	0.003	2.48	0.025			1.96	0.012	2.46	0
1987	fibrinogen beta chain	gi\|211780	612	100	53271.8	7.18	2.75	0.006							2.57	0.011
4679	glutathione S-transferase class-alpha	gi\|4959550	79	99.958	21326.5	8.03							1.36	0.005							1.8	0
4036	keratin, type I cytoskeletal 19	gi\|45384356	772	100	46111	4.94			2.43	0.016							2.15	0.009
5150	nmrA-like family domain-containing protein 1	gi\|71897147	653	100	32726	7.77							2.21	0.004							1.72	0.009
4405	PREDICTED: microfibril-associated glycoprotein 4 (EST)	gi\|18470900	331	100	28600	5.2			0.57	0.001							0.97	0.695
2021	Vimentin (VIM)	gi\|212868	1160	100	53166.6	5.09					0.76	0.015							0.56	0.001

a) Match ID represents unique numbers assigned to each spot in the matched standard of ImageMaster 2D Platinum version 6.0 software
b) Accession no. is the MASCOT result of MALDI-TOF/TOF searched from the NCBInr database
c) The criterion for a successfully identified protein is a protein score confidence interval (C.I. %) for PMF and MS/MS data ≥ 95%

ISSN: 1477-5956