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Table 2 Differential abundance changed proteins in tracheal from chicken infected with IBV ck/CH/LDL/97I P5 and ck/CH/LDL/97I P115

From: Proteomics analysis of differentially expressed proteins in chicken trachea and kidney after infection with the highly virulent and attenuated coronavirus infectious bronchitis virus in vivo

Match IDa

Protein description

Accession no.b

Protein score

Protein score CI%c

Mw (Da)

pI

Average ratio and p-value

       

P 5 -infected/control

P 115 -infected/control

       

4 dpi

7 dpi

14 dpi

21 dpi

4 dpi

7 dpi

14 dpi

21 dpi

       

ratio

p

ratio

p

ratio

p

ratio

p

ratio

p

ratio

p

ratio

p

ratio

p

2079,

2083

aldehyde dehydrogenase 2 family

gi|118098552

983

100

64491.8

8.79

1.55

0.001

      

2.72

0.09

      

2751

aldo-keto reductase

gi|14330324

795

100

36790.8

7.63

0.99

0.05

      

2.48

0.079

      

2382

alpha-enolase

gi|46048768

611

100

47617.5

6.17

4.87

0.019

      

2.28

0.91

      

2481,

4391

annexin A2 (ANXA2)

gi|45382533

783

100

38901

6.92

1.54

0.371

N/A

0

    

2.44

0.012

4.63

0.003

    

2224

cartilage matrix protein precursor

gi|71896317

1090

100

54798.1

6.63

12.82

0.019

      

2.59

0

      

1689

creatine kinase M-type

gi|211528

443

100

43529.1

6.5

  

0.87

0.567

      

5.63

0.001

    

4195,

4953

fructose-bisphosphate aldolase C

gi|999392

256

100

39022.8

5.79

  

0.34

0.009

  

0.26

0

  

2.25

0.009

  

2.23

0.002

3273

heat shock protein beta-1 (HSPB1)

gi|45384222

766

100

21715

5.77

5.42

0.005

      

2.66

0.955

      

4317

4310,

4321

lamin-A

gi|45384214

651

100

73348.5

6.5

      

2.88

0

      

0.41

0.001

2592

nmrA-like family domain-containing protein 1

gi|71897147

1090

100

32726

7.77

0.56

0.001

      

2.78

0.003

      

2483

similar to D4-GDP-dissociation inhibitor (GDID4)

gi|50728568

552

100

22928.6

5.08

    

2.37

0.039

      

1.61

0.254

  

1932

similar to malate dehydrogenase 2, NAD (mitochondrial)

gi|50758110

819

100

37400.6

8.83

  

N/A

0.019

      

1.3

0.31

    

5132

similar to myozenin

gi|50749396

319

100

29589.7

7.85

      

2.24

0.058

      

N/A

 

1919,

1659

pyruvate kinase (PKM2)

gi|212571

291

100

58433.9

7.29

1.12

0.488

0.44

0.071

0.5

0.012

  

2.55

0.004

2.81

0.024

1.28

0.088

  

4635

selenium binding protein 1

gi|118102241

731

100

53097.6

6.17

      

0.82

0.156

      

1.86

0.034

2016

succinyl-CoA:3-ketoacid-coenzyme A transferase 1, mitochondrial

gi|60592998

201

100

56549.4

8.01

5.49

0.138

      

2.09

0

      

4680

UMP-CMP kinase

gi|71896025

947

100

22386.3

6.75

  

1.07

0.56

      

2.42

0.002

    

4786

vimentin (VIM)

gi|212868

1210

100

53166.6

5.09

      

4.78

0

      

1.4

0.394

  1. a) Match ID represents unique numbers assigned to each spot in the matched standard of ImageMaster 2D Platinum version 6.0 software
  2. b) Accession no. is the MASCOT result of MALDI-TOF/TOF searched from the NCBInr database
  3. c) The criterion for a successfully identified protein is a protein score confidence interval (C.I. %) for PMF and MS/MS data ≥ 95%
  4. N/A: A indicates the spot was detectable on one of the gels, N indicates the spot was too weak to detect on one of the gels