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Table 3 Similar abundance changed proteins in kidney from chicken infected with IBV ck/CH/LDL/97I P5 and ck/CH/LDL/97I P115

From: Proteomics analysis of differentially expressed proteins in chicken trachea and kidney after infection with the highly virulent and attenuated coronavirus infectious bronchitis virus in vivo

Master IDa

Protein description

Accession no.b

Protein score

Protein score CI%c

Mw (Da)

pI

Average ratio and p-value

       

P 5 -infected/control

P 115 -infected/control

       

4 dpi

7 dpi

14 dpi

21 dpi

4 dpi

7 dpi

14 dpi

21 dpi

       

ratio

p

ratio

p

ratio

p

ratio

p

ratio

p

ratio

p

ratio

p

ratio

p

2033

carbonyl reductase [NADPH] 1

gi|71895267

1110

100

30519.9

8.5

            

1.66

0.005

  

435

catalase

gi|53127216

529

100

60279

8.09

0.64

0.002

      

0.67

0

      

252, 264

chain A, the structure of chicken mitochondrial Pepck in complex with Pep (PCK2)

gi|158430534

989

100

68009.9

6.55

4.27

0.015

      

3.6

0.019

    

1.56

0.038

2125,

3724

chain A, transthyretin

gi|1633502

672

100

14209

5.1

  

0.63

0.005

0.99

0.88

    

0.83

0.065

0.64

0

  

658

chain C, crystal structure of native chicken fibrinogen with two different bound ligands

gi|21730885

659

100

47400.8

5.32

  

1.71

0.039

      

1.68

0.022

    

1306

class I alcohol dehydrogenase, beta subunit

gi|45384164

286

100

40891.8

7.85

    

1.49

0.001

      

1.54

0.001

  

2087

cytochrome c oxidase subunit 4 isoform 1, mitochondrial

gi|71895513

359

100

19676.1

8.91

  

0.58

0.008

      

0.57

0.026

    

1274

D-amino-acid oxidase

gi|118098567

298

100

41209.8

6.7

    

1.4

0.15

      

1.55

0.001

  

144

ovotransferrin BB type

gi|71274075

1020

100

79606.1

6.85

1.3

0.003

      

1.56

0

      

374

similar to CDNA sequence BC048390

gi|118098312

1030

100

65576.4

7.85

0.64

0.001

      

0.60

0

      

417

similar to CDNA sequence BC048390

gi|118098312

734

100

65576.4

7.85

  

0.52

0.008

      

0.81

0.13

    

422

similar to CDNA sequence BC048391

gi|118098312

865

100

65576.4

7.85

  

1.35

0.033

      

1.52

0.021

    

436

similar to cystathionase

gi|118094764

618

100

44554.7

6.86

0.78

0.032

      

0.66

0.001

      

2149

similar to dodecenoyl-coenzyme A delta isomerase (3,2 trans-enoyl-coenzyme A isomerase)

gi|118098151

502

100

34557.8

9.3

    

1.63

0.003

      

1.19

0.023

  

670

similar to FLJ20699 protein

gi|118083181

352

100

52820.8

5.95

      

0.75

0.006

      

0.5

0

1527,

2150

similar to guanidinoacetate N-methyltransferase

gi|118103242

424

100

17457.7

6.59

0.63

0

  

0.65

0.001

  

0.73

0.001

  

0.86

0.002

  

1174

receptor-associated protein

gi|2661436

440

100

40792.2

8.61

    

1.66

0.032

      

1.28

0.11

  

511

retinal dehydrogenase 1

gi|45383031

534

100

56400.8

7.49

  

1.26

0.096

      

1.57

0.013

    

1739

similar to methyltransferase 24

gi|50750103

614

100

30640.5

6

      

1.65

0

      

1.87

0

  1. a) Match ID represents unique numbers assigned to each spot in the matched standard of Ettanâ„¢ DeCyder 2D v6.5 software
  2. b) Accession no. is the MASCOT result of MALDI-TOF/TOF searched from the NCBInr database
  3. c) The criterion for successful identification of a protein is a protein score confidence interval (C.I. %) for PMF and MS/MS data ≥ 95%