Figure 1

Phenotypes of OSRK1 transgenic rice. (A) PCR analysis of OSRK1 transgenic rice. Insertion and expression of the transgene was confirmed by genomic PCR (upper) and RT-PCR analysis (bottom). The PCR primers for OSRK1 were designed to specifically detect transgene or mRNA. RT-PCR analysis indicated that transcript level of OSRK1 was greatly increased in the transgenic rice roots. (B) Comparison of shoot and root growth of OSRK1 transgenic rice. Root and shoot length was measured after 9 days-growth on 1/2 MS agar media. (C) Effect of NaCl on the primary root growth of wild type and transgenic rice roots. Germinating seedlings with equally growing primary roots were selected and primary root length was measured 5 days after NaCl treatment at indicated concentrations. (D) In-gel kinase assay. Total soluble proteins were extracted from the roots of wild type or OSRK1 transgenic rice seedlings after 150 mM NaCl treatment for the indicated time period. Histone was used as a substrate.