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Table 1 Identification and quantitative analysis of early salt-responsive proteins in wild type and OSRK1 transgenic rice roots

From: Comparative proteomic analysis of early salt stress-responsive proteins in roots of SnRK2 transgenic rice

Spota

Protein Identification

Matched

peptide

Sequence Coverage

(%)

Mrb

(Ex/Tr)

NCBI

Accession

Number

Fold changec

Protein ratiod

(OSRK1/WT)

Express

Graphe

      

WT

OSRK1

  
      

3 h

7 h

3 h

7 h

  

Translation and transcription

          

5i

Elongation factor Tu

9

26.3

43.7/48.4

gi|21685576

ndf

7.32

2.47

1.19

Ig

12i

Putative translation elongation factor

eEF-1 beta chain

5

35.3

30.0/23.8

gi|113612079

0.97

2.00

1.92

0.55

1.25

323

Glycine-rich RNA-binding protein

6

74.0

13.1/15.5

gi|115441831

1.02

0.38

1.14

0.85

0.93

383

Translation initiation factor 5A

2

25.0

17.7/17.4

gi|113611710

nd

2.84

1.04

0.62

Ig

Amino acid and purine metabolism

26

Putative inosine monophosphate dehydrogenase

2

4.70

57.4/51.9

gi|215694434

0.38

1.25

0.89

0.88

1.05

27

Aspartate-semialdehyde dehydrogenase

3

10.9

40.1/40.1

gi|113549818

2.11

3.05

1.15

0.80

5.46

67

Aspartate aminotransferase

4

13.0

40.6/44.8

gi|29468084

1.48

4.51

0.87

0.97

3.64

68

Putative isovaleryl-CoA dehydrogenase

1

2.70

41.5/44.5

gi|113578072

0.88

1.89

0.91

0.89

2.53

200

Glutamine synthetase

4

10.4

41.4/39.2

gi|124052115

1.51

1.37

0.98

1.02

1.72

259

Methylmalonate semi-aldehyde dehydrogenase

5

10.5

18.1/57.2

gi|113610618

0.46

0.60

1.22

0.67

0.27

314

Aspartate aminotransferase

6

18.8

42.2/44.9

gi|215768565

1.13

3.14

0.87

1.06

3.08

314

Glutamate dehydrogenase

7

21.4

42.2/44.3

gi|33242905

1.13

3.14

0.87

1.06

3.08

 

Detoxyfying enzymes

51

Glutathione reductase, cytosolic

11

24.8

55.6/53.4

gi|113538016

0.63

2.80

0.89

1.01

1.82

112i

Gutathione S-transferase

4

 

25.6/25.6

gi|31433227

1.67

0.72

1.35

1.51

0.28

116

Ascorbate peroxidase

5

33.6

25.8/27.1

gi|50920595

1.79

1.13

0.80

1.10

2.40

125

Glutathione S-transferase II

6

32.1

26.0/24.0

gi|3746581

2.05

1.42

0.94

0.90

2.63

160i

Ascorbate peroxidase

10

46.0

24.0/27.1

gi|1321661

1.35

0.38

1.51

0.71

0.41

198

Quercetin 3-O-methyltransferase

2

6.8

41.4/39.7

gi|113623000

1.57

1.19

1.06

0.84

1.83

200

Peroxidase

3

8.6

41.4/37.8

gi|257657027

1.51

1.37

0.98

1.02

1.72

202

Similar to glyoxalase II

6

28.2

29.9/33.1

gi|113533338

1.07

1.98

0.94

0.90

1.47

206

Glyoxalase I

10

51.5

33.9/32.5

gi|113623141

3.57

1.73

0.84

1.00

4.46

213

Thioredoxin Type H

7

50.8

13.6/13.9

gi|82407383

1.94

0.87

1.01

0.98

3.20

265i

Similar to glyoxalase II

7

32.5

32.5/33.2

gi|113533338

0.59

1.52

0.68

0.74

1.90

310

Ascorbate peroxidase

5

38.2

25.6/27.1

gi|257707656

0.79

0.34

1.85

4.40

0.10

351

Ascorbate peroxidase

4

24.8

26.7/27.1

gi|1321661

1.98

0.65

1.00

0.97

1.35

390

Aldehyde dehydrogenase

3

8.0

54.6/59.2

gi|8163730

3.16

3.10

0.85

1.25

10.85

Glycolysis and other carbohydrate metabolism related proteins

61

Fructose -bisphosphate aldolase

3

9.8

29.3/38.8

gi|790970

0.26

0.94

0.69

0.81

0.82

67

Phosphoglycerate kinase

3

11.0

40.6/42.3

gi|113596357

1.48

4.51

0.87

0.97

3.64

76i

Dihydrolipoamide dehydrogenase precursor

5

13.5

60.6/52.6

gi|113532449

nd

16.87

0.87

1.54

Ig

77

Transketolase

7

10.6

76.8/80.0

gi|227468492

2.14

15.09

1.02

0.98

18.68

79

Similar to enolase

6

15.9

52.0/50.7

gi|115478881

1.90

0.78

0.80

0.74

3.14

116

Triose phosphate isomerase

13

79.1

25.8/27.6

gi|553107

1.79

1.13

0.80

1.10

2.40

275

2,3-Bisphosphoglycerate-independent phosphoglycerate mutase

8

27.2

70.8/60.8

gi|257353836

1.45

2.56

0.80

0.84

2.07

277

2,3-Bisphosphoglycerate-independent phosphoglycerate mutase

3

12.2

70.8/60.8

gi|257353836

1.26

2.83

0.86

0.88

2.33

279i

Enolase

4

14.4

57.0/47.9

gi|113548027

1. 03

1.70

0.72

0.80

1.41

292

Phosphoglyceromutase

9

20.8

69.9/60.7

gi|257353838

1.03

2.13

0.93

1.04

2.66

293

Phosphoglyceromutase

12

28.1

69.9/60.7

gi|257353838

1.50

1.79

0.87

1.02

1.90

315

Glyceralde-3-phosphate dehydrogenase

4

16.3

39.1/36.5

gi|968996

0.99

2.14

0.91

1.10

2.15

318

Glyceralde-3-phosphate dehydrogenase

4

16.3

38.7/36.4

gi|968996

1.17

2.68

1.03

1.01

3.06

353

Triose phosphate isomerise

4

20.9

22.0/27.0

gi|169821

0.83

0.62

1.16

0.67

0.32

385

Phosphoglycerate kinase

5

16.7

45.4/42.2

gi|113596357

nd

4.57

1.03

1.10

Ig

Proteolytic enzymes

112i

Proteasome subunit beta type 2

5

26.4

25.6/23.4

gi|17380213

1.67

0.72

1.35

1.51

0.28

134

Oryzacystatin

3

23.5

13.3/11.4

gi|1280613

2.10

0.46

0.87

1.00

0.22

204

Proteasome subunit alpha type 2

7

34.6

26.2/27.6

gi|259443357

0.59

1.88

0.89

1.15

1.22

351

Proteasome subunit alpha type 2

4

16.6

26.7/25.8

gi|259443327

1.98

0.65

1.00

0.97

1.35

Heat shock proteins

112i

Putative chaperonin21 precursor

5

26.2

25.6/26.3

gi|51090748

1.67

0.72

1.35

1.51

0.28

274

Chaperonin CPN60-1, mitochondrial precursor

10

17.5

64.1/61.0

gi|113547409

1.17

2.77

0.88

1.12

1.49

276i

DnaK-type molecular chaperone precursor

2

4.6

75.0/70.4

gi|257307253

1.26

3.35

0.76

1.00

2.78

278i

DnaK-type molecular chaperone precursor

6

14.5

72.9/70.4

gi|257307253

2.35

2.38

0.66

1.47

4.47

Lipid biosynthesis

258

Putative enoyl-ACP reductase

3

15.7

35.4/39.1

gi|113623526

1.32

2.41

1.13

1.01

2.92

69

Putative acetyl-CoA C-acetyltransferase

6

19.2

43.1/44.1

gi|113630918

1.28

3.46

0.80

0.99

6.40

82

Putative inorganic pyrophosphatase

5

21.4

31.8/33.0

gi|113537770

0.88

0.41

0.97

0.99

0.42

177

Nucleoside diphosphate kinase from rice

2

16.6

14.5/16.8

gi|113639936

0.39

0.81

0.88

0.83

1.14

227i

Putative inorganic pyrophosphatase

5

13.9

32.1/33.0

gi|113537770

1.70

0.41

0.92

1.30

0.44

Stress related proteins

124

Formate dehydrogenase, mitochondrial precursor

13

47.2

42.4/41.2

gi|21263611

0.64

7.24

0.78

0.90

4.13

134

15 kda Organ-specific salt-induced protein

2

23.4

13.3/15.2

gi|256638

2.10

0.46

0.87

1.00

0.22

214

15 kda Organ-specific salt-induced protein

7

77.2

13.4/15.2

gi|256638

4.05

0.33

0.87

1.12

0.48

227i

15 kda Organ-specific salt-induced protein

6

64.8

32.1/15.2

gi|256638

1.70

0.41

0.92

1.30

0.44

386

Pathogen-related protein

4

21.3

16.5/17.2

gi|16589076

4.29

2.28

1.30

0.38

6.70

Signal transduction related proteins

225

Calreticulin

3

5.9

33.4/47.9

gi|6682833

0.75

0.43

1.47

1.26

0.26

Unkwown proteins

392

Hypothetical protein

3

10.7

25.8/27.4

gi|14192878

5.65

4.00

0.79

0.96

18.95

  1. aSpot numbers correspond to the spots in Figure 2 and Figure 3
  2. bMolecular weight (kDa) of protein spots estimated from gel analysis (Ex) and theoretical molecular weight of identified protein (Tr)
  3. cFold change of each spot after NaCl treatment for 3 h or 7 h, calculated from the mean spot volumes in control and NaCl treated gel groups
  4. dSpot-volume ratio of WT to OSRK1, calculated from the mean spot volumes in 3 h control of WT and OSRK1 groups
  5. eRelative expression graphs of protein spots after NaCl treatment in WT and OSRK1. Spot volumes are analyzed by Progenesis software. The left four bars indicate spot volume of WT root and right four bars indicate spot volumes of OSRK1. From left to right, each bar indicate spot volume of 3 h control, 3 h NaCl treated, 7 h control and 7 h NaCl treated gel groups. Values are means ± SE. Error bars from three spots in three independent gels
  6. fnd: not detected
  7. g I: spots were not detected in WT groups, but appeared in OSRK1 groups
  8. hProteins not identified from the database
  9. i Salt-responsive proteome identified from OSRK1 transgenic rice roots
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Proteome Science

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