1 | 2 | 3 | 3 + DTT | 3 + ASB-14 | ||
Extraction | Tris–HCl pH 7,6 | 40 mM | - | - | - | - |
Tris | - | 20 mM | - | - | - | |
Urea | - | 9 M | 7 M | 7 M | 7 M | |
Tiourea | - | - | 2 M | 2 M | 2 M | |
EDTA | - | 1 mM | - | - | - | |
CHAPS (w/v) | 2% | 4% | 4% | 4% | 4% | |
Triton X-100 (v/v) | - | - | 0.5% | 0.5% | 0.5% | |
DTT | - | 10 mM | - | 100 mM | - | |
ASB-14 (w/v) | - | - | - | - | 2% | |
Precipitation | No | No | Yes | Yes | Yes | |
Rehydration | Tiourea | 2 M | - | 2 M | ||
Urea | 7 M | 9 M | 7 M | |||
DTT | 50 mM | 18 mM | 100 mM | |||
CHAPS (w/v) | 2% | 2% | 4% | |||
Triton x-100 (v/v) | - | - | 0.5% | |||
IPG Buffer pH 3-10 (v/v) | 0.5% | 2% | 0.2% | |||
Bromofenol blue | traces | traces | traces |