Table 1 Sample preparation procedures and buffers composition

1

2

3

3 + DTT

3 + ASB-14

Extraction

Tris–HCl pH 7,6

40 mM

-

-

-

-

Tris

-

20 mM

-

-

-

Urea

-

9 M

7 M

7 M

7 M

Tiourea

-

-

2 M

2 M

2 M

EDTA

-

1 mM

-

-

-

CHAPS (w/v)

2%

4%

4%

4%

4%

Triton X-100 (v/v)

-

-

0.5%

0.5%

0.5%

DTT

-

10 mM

-

100 mM

-

ASB-14 (w/v)

-

-

-

-

2%

Precipitation

No

No

Yes

Yes

Yes

Rehydration

Tiourea

2 M

-

2 M

Urea

7 M

9 M

7 M

DTT

50 mM

18 mM

100 mM

CHAPS (w/v)

2%

2%

4%

Triton x-100 (v/v)

-

-

0.5%

IPG Buffer pH 3-10 (v/v)

0.5%

2%

0.2%

Bromofenol blue

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