Figure 4

The amino acid sequence of a very acidic protein, Lotgi1|238358. Entry Lotgi1|238358 contains the sequence of a predicted transmembrane protein with a short intracellular domain (aa2–20), the predicted transmembrane segment (underlined) and a very acidic extracellular domain (theoretical pI 3.6) with Asp and Glu adding up to 30% of the amino acid composition. This protein was more abundant in the acid-insoluble than in the acid-soluble fraction. Sequences covered by MS/MS spectra a shown in red. The lower part shows the spectrum of one of the acidic, doubly charged peptides (shown in bold italics and underlined in the complete sequence) with m/z 831.3731, a mass error of 1.4 ppm and a PEP of 1.1E-12.