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Table 1 Previously uncharacterized major Lottia shell matrix proteins with unusual primary sequence features

From: In-depth proteomic analysis of a mollusc shell: acid-soluble and acid-insoluble matrix of the limpet Lottia gigantea

Accession

Feature

Lotgi1|115147

14% P, 11% T, 6 repeats of ~30aa, starting with MITPE; pI: 4.7; 319aa

Lotgi1|142790

25% Q, 10% E, 17% P, 12% V, 10% N 10% L; 6 short repeats: k/qQQPxVELNKQQP; pI 5.2; 182aa

Lotgi1|142814

38% Q, 11% L, 10% P; 5 ~70aa repeats containing shorter repeat motifs like NQQQ and KQQQ; pI: 10.5; 322aa

Lotgi1|152688

20% G, 12% P; pI: 9.7; 137aa

Lotgi1|158113

11% P; Q-rich C-term (aa210–240); pI: 9.7; 258aa

Lotgi1|159331

26% E, 13% L,12% T; pI: 4; starting with aa156 8x SNLLQQPDa/tTQqLa/tTNeQQQ; (Figure 6 )

Lotgi1|163637

17% D, 16% A; EFh, pI: 3.8; 643aa; 12 ca30aa repeats similar to AxVDNxxMADMIDTxQDxxEDAADNMADNIDTAQDAQ between aa32–453

Lotgi1|171084

13% S; frequent doublets (SS, QQ, TT, YY, NN); G/E block aa322–337; pI: 4.4; 357aa

Lotgi1|172698

23% Q, 13% N, 13% S; aa130–702: 31 x 14aa repeats similar to QSNQQFNxxQSNQQF; pI: 7.1; 1184aa

Lotgi1|173200

~10% of P, N and G; in aa107–170 10x GAMP/GSMP; pI: 9.6; 563aa

Lotgi1|174003

19% P in aa50–400 and 35% P in aa778–882; pI: 9.5; 882aa

Lotgi1|227783

aa17–126: 17% R + K, 12% P, 11% L; pI: 11; 126aa

Lotgi1|228385

16% R, 11% S; pI: 11.7; 160aa; R/H-rich from aa103–150

Lotgi1|231186

19% G, 12% P; aa433–481: 27% M; pI: 4.6; 481aa; R/H-rich C-term half

Lotgi1|231509

aa26–230: 18% P; pI: 4.2; 230aa; acidic blocks in N-term half

Lotgi1|233397

A/P-rich motif aa150–170; H-rich motif aa171–185; pI: 8.8; 219aa

Lotgi1|233420

31% D, 10% E; pI: 3.6; similar to aspein?

Lotgi1|234884

42% Q in aa281–630; G/L/A-rich region aa631–928; pI: 9.2; 928aa

Lotgi1|235497

aa120–247: 20% P, 16% A, 10% Q; pI: 9.7; 247aa

Lotgi1|235610

15% P, 15% T; pI:5.7; 557aa

Lotgi1|235621

aa171–270: 33% G, 25% T, 15% P, 14% Q; 16 x GGQPs/tT; pI: 5.4; 303aa

Lotgi1|235812

24% P, 18% Q, 10% N; pI: 8.9; 729aa; aa57–376: 17 repeats of 16aa, similar to NNxa/vQPPxxQxxYQPt/p

Lotgi1|236689

19% P, 10% A, 10% V, 10% R; pI: 10; 317aa

Lotgi1|236690

21% Q, 18% P; aa268–356: 4 xAQPGAYQQP(x)2–4 GAYxQQP repeats; pI: 8.4; 440aa

Lotgi1|236691

22% P, 13% Q, 10% A; Q-rich regions: ~aa61–160 and ~ aa721–990; P-rich: ~aa280–600 and ~780–970¸pI: 8.8; 1035aa

Lotgi1|238358

aa61–232: 32% D + E, 12% N; pI: 3.7; 323aa; (Figure 4 )

Lotgi1|238831

13% A, 11% R, 11% L; K/R/A-rich C-terminus (aa185–219); pI. 10.3; 219aa

Lotgi1|239170

16% G, 12% M, 10% Q; G blocks in N-term half; pI: 9.9; 145aa

Lotgi1|239174

20% G, 18%M, 12%A, 10% L; pI: 11.2; 186aa; some similarity to shematrins

Lotgi1|239339

13% T, 12% S, 10% P; blocks of T from aa185–240; pI: 9.7; 609aa

Lotgi1|239447

22% G, 12% N; pI:9.5; 191aa; some similarity to GAAP_HALAI (Figure 5 )

Lotgi1|77105

19% P, 15% S; 12% G; 9 x g/dSQPGIYP and 4 x imperfect; pI: 4.5; 173aa

Lotgi1|84059

23% N, 15% P, 15%T, 11% S; 7 repeats similar to TPxxxNNVNPGSETPxTxNNVNPGSE and 2 incomplete; pI: 3.8; 234aa

  1. For complete lists of matrix proteins see Additional file 1 and Additional file 2. Accessions in bold belong to the 26 most abundant proteins with average emPAI >1000 ( Additional file 1 and Additional file 2).