Figure 1

Composite images of 2D gels of proteins extracted from liver of control, chronic ethanol, control-binge, and chronic ethanol-binge treated rats. Rats were fed ethanol in liquid diet chronically for 4 weeks and then given three ethanol binges (5 g/kg) at 12 hr intervals. Four hours after the last dose liver samples were frozen in liquid nitrogen and stored for proteome studies. Proteins were extracted by phenol and methanol/ammonium acetate precipitation and 2D electrophoresis and analysis were performed as described under Materials and Methods. Figure 1 represents a composite image of the gel and the proteins that are differentially expressed between the treatment groups and the protein sequenced are shown by spot numbers. Control represents pair –fed animals that were given water for binge control. N = 3 rats for each group.