Figure 7

Parameters of liver injury and histology after chronic and chronic ethanol- three binge. Rats were fed ethanol in liquid diet chronically for 4 weeks and then given 3 binge (5 gm/kg) at 12 hr intervals. Four hours after the last dose, the levels of serum ALT, and hepatic cleaved caspase-3 were determined as described under Materials and Methods. Sections of liver samples were stained with hematoxylin and eosin. Control represents pair –fed animals and were given water for binge control. Values are mean ± SE (n = 3 rats). Western blot represents a typical experiment. A-D: Hemotoxylin and eosin staining (x 200X); E: CYP2E1 protein expression. F: 4-HNE immunoblot G: Serum ALT; H: hepatic cleaved caspase 3; I: H2AX phosphorylation a: significant from control group (p < 0.05); C: Control (pair fed); E: Chronic ethanol; C-B: Control- ethanol binge; E-B: Chronic-ethanol binge. LMW: low molecular weight; HMW: high molecular weight; Small solid arrow represents leukocyte infiltration.