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Figure 4 | Proteome Science

Figure 4

From: Identification of differentially accumulated proteins associated with embryogenic and non-embryogenic calli in saffron (Crocus sativus L.)

Figure 4

2-DE map of proteins extracted from NEC after SE induction in vitro. Total soluble proteins were extracted by phenol extraction method; 160-μg protein was loaded into 18 cm IPG strips with linear pH gradient of 4-7 for isoelectric focusing (IEF). Second dimension was run in 11.5% SDS-PAGE gels. Proteins were visualized by silver staining. Arrows show the positions of the identified proteins by MS, which showed statistically significant change in abundance. The numbering corresponds to the match IDs as shown in table 1.

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