Figure 1

Analysis of purified NDV particles. (A) Electron micrograph of negatively stained with 2% potassium phosphotungstate (pH 6.5), sucrose density gradients purified NDV La Sota virus at 50,000 × magnification. (B) SDS-PAGE separation of proteins in purified NDV particles. 15 μg total proteins were separated on a 5-15% polyacrylamide gel and stained with coomassie blue. The positions of the viral proteins identified by their predicted molecular weights were indicated. M: protein marker, V: purified NDV, HN: Haemagglutinin Neuraminidase, F: Fusion protein, NP: Nucleocapsid protein, P: Phosphate protein, M: Matrix protein.