Figure 3
From: Proteomic analysis of purified Newcastle disease virus particles
Confirmation of host proteins incorporated into purified NDV particles by Western blot. 8 μg of purified virions from allantoic fluid (AF) (lane 1) and 15 μg of proteins extracted either from the normal 13-day-old specific pathogen free (SPF) chicken embryo (CEL) (lane 2) or from the AF of the uninfected 13-day-old specific pathogen free (SPF) chicken embryo eggs (lane 3) were subjected to western blot analysis with antibodies against the following proteins: (A) HN, (B) Hsp90, (C) Actin, (D) Tubulin alpha-1, (E) Annexin A2. Numbers to the left are molecular weight markers (M).