Figure 4
SPP interacts with vigilin. (A) Co-immunoprecipitation with anti-SPP-CT antibody pulled down endogenous vigilin. The blot was probed with anti-vigilin antibody. HEK293 cell lysate was used to align the band. (B) In a HEK293 cell line stably expressing FLAG-tagged vigilin, co-immunoprecipitation with anti-SPP-CT antibody pulls-down vigilin-FLAG, while antibody against SPPL2b did not pull down vigilin-Flag. The blot was probed with anti-FLAG antibody. (C) Co-immunoprecipitation with anti-FLAG antibody pulled-down SDS-stable SPP dimers and trimers in the FLAG-tagged vigilin expressing HEK293 cell line. The blot was probed with anti-SPP-CT antibody. Data shown are representative blots from three independent experiments.