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Table 1 List of proteins identified in the iTRAQ mass spectrometry analysis of SPP interacting proteins

From: Vigilin interacts with signal peptide peptidase

Protein Name

Accession Number

Symbol

Unique Peptides

Coverage (%)

iTRAQ Ratio (117:116)

Signal peptide peptidase

IPI:IPI00152441.3

SPP

6

22.0

331.3

Vigilin

IPI:IPI00022228.1

VIGLN

13

14.6

4.3

10 kDa heat shock protein, mitochondrial

IPI:IPI00220362.4

HSPE1

9

72.3

1.4

Microfibrillar-associated protein 1

IPI:IPI00022790.1

MFAP1

5

20.3

1.3

Heat shock protein 60

IPI:IPI00472102.3

HSP60

23

63.8

1.2

Calnexin precursor

IPI:IPI00020984.1

CNX

9

15.5

1.1

Alpha-enolase

IPI:IPI00465248.4

ENO1

14

37.2

1.1

Glyceraldehyde-3-phosphate dehydrogenase

IPI:IPI00219018.6

GAPDH

8

33.5

1.1

Protein C14orf166

IPI:IPI00006980.1

C14orf166

7

39.8

1.1

Stress-70 protein, mitochondrial precursor

IPI:IPI00007765.5

HSPA9

43

71.4

1.0

Eukaryotic translation initiation factor 4 gamma 1

IPI:IPI00552639.2

EIF4G1

8

13.6

1.0

ATP synthase O subunit, mitochondrial precursor

IPI:IPI00007611.1

ATP5O

10

59.1

1.0

Actin, cytoplasmic 1

IPI:IPI00021439.1

ACTB

9

47.5

1.0

130 kDa leucine-rich protein

IPI:IPI00329745.4

LPPRC

11

12.8

1.0

Sideroflexin-1

IPI:IPI00009368.3

SFXN1

8

31.8

0.9

ATP-dependent DNA helicase 2 subunit 1

IPI:IPI00644712.3

Ku70

11

20.7

0.9

Prohibitin

IPI:IPI00017334.1

PHB

8

34.2

0.9

Heat shock 70 kDa protein 1

IPI:IPI00643932.1

HSP70-1

29

49.3

0.9

Splice Isoform 2 of Nucleophosmin

IPI:IPI00220740.1

NPM1

8

41.5

0.9

Protein disulfide-isomerase

IPI:IPI00010796.1

PDIA3

24

30.7

0.9

ATP synthase beta chain, mitochondrial precursor

IPI:IPI00303476.1

ATP5B

9

29.7

0.9

14-3-3 protein zeta/delta

IPI:IPI00021263.3

YWHAZ

7

45.3

0.9

NADH-ubiquinone oxidoreductase 24 kDa subunit, mitochondrial precursor

IPI:IPI00646556.1

NDUFV2

7

38.5

0.9

Heat shock 70 kDa protein 5

IPI:IPI00003362.2

HSPA5

26

52.1

0.9

ATP-dependent RNA helicase A

IPI:IPI00215638.5

DHX9

12

16.4

0.8

Splice Isoform 1 of Heat shock cognate 71 kDa protein

IPI:IPI00003865.1

HSPA8

16

51.4

0.8

ATP-dependent RNA helicase DDX3X

IPI:IPI00215637.4

DDX3X

8

15.9

0.8

Vimentin

IPI:IPI00646867.1

VIM

12

28.1

0.8

Splice Isoform A1-A of Heterogeneous nuclear ribonucleoprotein A1

IPI:IPI00465365.3

HNRNPA1

12

64.6

0.8

protein kinase C substrate 80K-H isoform 1

IPI:IPI00419384.1

PRKCSH

11

23.7

0.8

Programmed cell death 8

IPI:IPI00157908.3

PDCD8

7

14.6

0.8

peroxiredoxin 3 isoform b

IPI:IPI00374151.1

PRDX3

8

34.0

0.8

FK506-binding protein 10

IPI:IPI00303300.3

FKBP10

8

21.6

0.8

Elongation factor 2

IPI:IPI00186290.5

EEF2

12

20.3

0.8

Reticulocalbin-1

IPI:IPI00015842.1

RCN1

6

19.6

0.8

Splice Isoform 1 of Apoptosis inhibitor 5

IPI:IPI00555572.1

API5

9

24.7

0.8

Citrate synthase, mitochondrial precursor

IPI:IPI00025366.4

CS

8

19.3

0.8

Transitional endoplasmic reticulum ATPase

IPI:IPI00478540.2

VCP

8

13.9

0.8

Single-stranded DNA-binding protein, mitochondrial precursor

IPI:IPI00029744.1

SSBP1

10

74.3

0.8

Fructose-bisphosphate aldolase A

IPI:IPI00465439.4

ALDOA

6

26.7

0.8

Creatine kinase B-type

IPI:IPI00022977.1

CKB

6

23.1

0.8

Neutral alpha-glucosidase AB precursor

IPI:IPI00472068.1

GANAB

8

16.0

0.8

14-3-3 protein epsilon

IPI:IPI00000816.1

YWHAE

9

51.4

0.7

Nuclease sensitive element-binding protein 1

IPI:IPI00031812.2

NSEP1

7

48.6

0.7

Spliceosome RNA helicase BAT1

IPI:IPI00328343.7

DDX39B

9

22.2

0.7

Nucleolin

IPI:IPI00743912.1

NCL

16

31.9

0.7

ADP/ATP translocase 2

IPI:IPI00007188.4

SLC25A5

9

35.4

0.7

Prohibitin-2

IPI:IPI00027252.6

PHB2

8

43.8

0.7

ADP/ATP translocase 3

IPI:IPI00291467.6

SLC25A6

9

35.4

0.7

Splice Isoform 2 of Heterogeneous nuclear ribonucleoprotein K

IPI:IPI00216746.1

HNRPK

12

41.8

0.7

peptidylprolyl isomerase B

IPI:IPI00646304.3

PPIB

7

40.7

0.7

Peroxiredoxin-1

IPI:IPI00000874.1

PRDX1

6

34.7

0.7

Mitochondrial precursor proteins import receptor

IPI:IPI00015602.1

TOMM70A

11

26.2

0.7

Splice Isoform 1 of Polyadenylate-binding protein 1

IPI:IPI00008524.1

PABPC1

14

29.2

0.7

Splice Isoform 3 of DNA-binding protein A

IPI:IPI00219148.2

CSDA

6

31.3

0.7

Splice Isoform 3 of Interleukin enhancer-binding factor 3

IPI:IPI00414335.1

ILF3

9

16.2

0.7

Splice Isoform 2 of Probable ATP-dependent RNA helicase DDX17

IPI:IPI00651677.1

DDX17

7

19.9

0.7

40S ribosomal protein S10

IPI:IPI00008438.1

RPS10

7

49.1

0.7

Elongation factor 1-alpha 1

IPI:IPI00472724.1

EEF1A1

8

22.5

0.7

Endoplasmin

IPI:IPI00027230.3

HSP90B1

23

32.7

0.7

Dihydrolipoyllysine-residue succinyltransferase component of 2- oxoglutarate dehydrogenase complex, mitochondrial precursor

IPI:IPI00420108.4

DLSTP1

9

31.8

0.7

Splice Isoform B1 of Heterogeneous nuclear ribonucleoproteins A2/B1

IPI:IPI00396378.3

HNRNPA2B1

19

57.5

0.7

Splice Isoform Long of Splicing factor, proline- and glutamine-rich

IPI:IPI00010740.1

SFPQ

26

50.2

0.7

CKAP4 protein (Fragment)

IPI:IPI00433214.1

CKAP4

16

34.3

0.6

Calreticulin

IPI:IPI00020599.1

CALR

6

25.2

0.6

Protein disulfide-isomerase A6

IPI:IPI00644989.1

PDIA6

9

27.7

0.6

ATP synthase alpha chain, mitochondrial precursor

IPI:IPI00440493.2

ATP5A1

15

36.7

0.6

Nucleobindin-2

IPI:IPI00009123.1

NUCB2

5

23.6

0.6

40S ribosomal protein SA

IPI:IPI00553164.3

RPSA

7

39.1

0.6

Splice Isoform C1 of Heterogeneous nuclear ribonucleoproteins C1/C2

IPI:IPI00216592.1

HNRNPC

12

52.9

0.6

Heterogeneous nuclear ribonucleoprotein G

IPI:IPI00304692.1

RBMX

16

49.1

0.6

Serine hydroxymethyltransferase, mitochondrial precursor

IPI:IPI00002520.1

SHMT2

7

17.9

0.6

Protein disulfide-isomerase A4

IPI:IPI00009904.1

PDIA4

15

35.7

0.6

Proliferation-associated protein 2G4

IPI:IPI00299000.4

PA2G4

14

36.6

0.5

Non-POU domain-containing octamer-binding protein

IPI:IPI00304596.3

NONO

18

42.0

0.5

Histone H1.2

IPI:IPI00217465.4

HIST1H1C

5

34.0

0.5

Complement component 1, Q subcomponent-binding protein, mitochondrial precursor

IPI:IPI00014230.1

C1QBP

6

42.9

0.5

THO complex subunit 4

IPI:IPI00328840.7

THOC4

7

37.1

0.4

  1. Vigilin or SPP-derived peptides were selectively enriched in the SPP purification sample compared to the control purification, while all other proteins identified had iTRAQ 117:116 ratios of less than 1.5.