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Table 1 Observed basic pI shifts in OGE fractionation after iTRAQ labelling

From: Optimization of iTRAQ labelling coupled to OFFGEL fractionation as a proteomic workflow to the analysis of microsomal proteins of Medicago truncatula roots

 

Sequence

Mass NL

Mass L

Difference

F NL

F L

1

ECADLWPR

1045.46

1190.57

145.11

1

3,4

2

AYLEDFYR

1075.50

1220.61

145.11

1

3,4

3

EQMGYTFDALK

1301.58

1590.81

289.23

1

3,4

4

TMADEGVVALWR

1346.65

1491.77

145.12

1,2

3,4

5

ECSGVEPQLWAR

1430.66

1575.77

145.11

1

3,4

6

NQIDEIVLVGGSTR

1499.78

1644.90

145.13

1

3,4

7

LAEMPADSGYPAYLAAR

1794.84

1939.97

145.13

1

3,4

8

ELEFYMK

958.46

1247.66

289.20

1

3

9

IPSAVGYQPTLSTDLGGLQER

2201.13

2346.24

145.11

1

3,4

10

AQIWDTAGQER

1273.59

1418.71

145.12

1

3,4

11

AGGECLTFDQLALR

1549.77

1694.87

145.10

1

3,4

12

VDFAYSFFEK

1251.61

1540.79

289.18

1

3

13

IFDKPEDFIAER

1478.74

1767.95

289.22

1

3,4

14

GLFTSDQILFTDTR

1612.79

1757.92

145.13

1

3,4

15

TTPSYVAFTDSER

1472.66

1617.79

145.13

1

3,4

16

LDTGNFSWGSEAVTR

1638.76

1783.87

145.11

1

3,4

17

LWQVPETLPAEVVGK

1664.93

1954.13

289.19

2

3,4

18

QLDAHIEEQFGGGR

1555.73

1700.85

145.11

2

3,4

19

GFGFVTFAEEK

1230.61

1519.80

289.19

2

3,4

20

AFLVEEQK

962.51

1251.72

289.21

2

3,4

21

IFEGEALLR

1046.59

1191.69

145.10

2

3,4

22

ISGLIYEETR

1179.61

1324.72

145.11

2

3,4

23

TTAEEGVVALWR

1330.71

1475.80

145.09

2

3,4

24

LLIQNQDEMIK

1343.72

1632.92

289.21

2

3,4

25

IQDKEGIPPDQQR

1522.77

1811.99

289.22

2

3,4

26

TMVYPEAGFELQR

1539.74

1684.85

145.10

2

3,4

27

EQDVSLGANKFPER

1588.77

1878.00

289.22

2

3,4

28

GQGGIQQLLAAEQEAQR

1795.93

1941.03

145.10

2

3,4

29

QYAVFDEK$

981.46

1287.68

306.22

2

3,4

30

QLDSHIEEQFGGGR$

1554.71

1716.84

162.13

2

3,4

31

FDVGVKEIEGWTAR

1605.84

1895.03

289.19

2

3,4

32

HFEVDLSAFR

1219.61

1364.71

145.10

3

4

33

CALVYGQMNEPPGAR

1661.78

1806.87

145.10

4

6

34

NAVVTVPAYFNDSQR

1679.84

1824.94

145.10

4

5,6

35

QPTELELAQAFHQGK

1695.85

1985.07

289.22

4

5

36

TALTYVDNNDGSWHR

1747.80

1892.90

145.10

4

5

37

QQFPLALYQVDK

1448.78

1737.98

289.20

4

5,6

38

ADGFAGVFPEHK

1273.60

1562.82

289.22

4

5

39

SLEGLQANVQR

1213.63

1358.75

145.13

4

6

40

TFDNVYYK

1048.47

1337.70

289.23

4

5

41

MLSPLILGDEHYQTAR

1842.96

1988.04

145.08

4

5

42

SSFDAFQQILK

1282.67

1571.87

289.20

4

5

43

SSDFLMYGIK

1159.57

1448.77

289.20

4

5

44

SSMDAFQQILK

1282.67

1571.83

289.17

5

5

45

FTQANSEVSALLGR

1491.77

1636.88

145.11

5

6

46

STLVWEVR

988.56

1133.64

145.08

5

6

47

IFLENVIR

1002.62

1147.70

145.07

5

6

48

FFCEFCGK

1093.48

1382.65

289.17

5

6

49

EVAGFAPYEKR

1265.65

1554.85

289.20

5,6

6

50

SFGPAVIFNNEK

1321.70

1610.88

289.18

5

6

51

VALINYGPEYGR

1350.75

1495.80

145.06

5

6

52

YIAPEQVPVK

1142.63

1431.85

289.21

5

5,6

53

VEPLVNMGQITR

1355.72

1500.83

145.12

5

6

54

AYEPILLLGR

1143.67

1288.77

145.10

5

6

55

LVGEYGLR

905.51

1050.61

145.10

5

6

56

EALGGLPLYQR

1215.66

1360.77

145.11

5

6

57

ADAFLLVGTQPR

1286.70

1431.81

145.10

5

6,7

58

HGWEYVVK

1016.51

1305.72

289.21

6

8

59

FVIGGPHGDAGLTGR

1452.75

1597.86

145.10

7

8

60

LVNVFTIGK

989.60

1278.80

289.20

7

9,10

61

THAVVEPFVIATNR

1552.86

1697.95

145.09

7

8,9

62

SVHEPMQTGLK

1225.62

1514.82

289.21

7

8

63

SVVYALSPFQQK

1365.74

1654.94

289.20

7

10

64

YGGGANFVHDGYNK

1497.61

1786.88

289.27

7

8

65

TALTYIDGNGNWHR

1616.76

1761.88

145.12

8

9

66

AHLQDYIQTHYTAPR

1812.89

1958.00

145.11

8

9

67

NYTNAFQALYR

1359.66

1504.77

145.11

10

12

68

TLHPNWSPAAIK

1333.72

1622.93

289.21

10

11

69

FHQYQVVGR

1132.56

1277.69

145.13

11

12

70

FQSLGVAFYR

1186.64

1331.72

145.08

11

12

  1. This table presents 70 peptides shifted to more basic OGE fraction after iTRAQ labelling. The non-labelled (NL) and iTRAQ labelled (L) peptide masses are shown in this table together with the mass difference induced by the labelling. The OGE fractions (F) of iTRAQ labelled peptides and their native counterparts are as well presented.