Skip to main content

Table 1 Observed basic pI shifts in OGE fractionation after iTRAQ labelling

From: Optimization of iTRAQ labelling coupled to OFFGEL fractionation as a proteomic workflow to the analysis of microsomal proteins of Medicago truncatula roots

  Sequence Mass NL Mass L Difference F NL F L
1 ECADLWPR 1045.46 1190.57 145.11 1 3,4
2 AYLEDFYR 1075.50 1220.61 145.11 1 3,4
3 EQMGYTFDALK 1301.58 1590.81 289.23 1 3,4
4 TMADEGVVALWR 1346.65 1491.77 145.12 1,2 3,4
5 ECSGVEPQLWAR 1430.66 1575.77 145.11 1 3,4
6 NQIDEIVLVGGSTR 1499.78 1644.90 145.13 1 3,4
7 LAEMPADSGYPAYLAAR 1794.84 1939.97 145.13 1 3,4
8 ELEFYMK 958.46 1247.66 289.20 1 3
9 IPSAVGYQPTLSTDLGGLQER 2201.13 2346.24 145.11 1 3,4
10 AQIWDTAGQER 1273.59 1418.71 145.12 1 3,4
11 AGGECLTFDQLALR 1549.77 1694.87 145.10 1 3,4
12 VDFAYSFFEK 1251.61 1540.79 289.18 1 3
13 IFDKPEDFIAER 1478.74 1767.95 289.22 1 3,4
14 GLFTSDQILFTDTR 1612.79 1757.92 145.13 1 3,4
15 TTPSYVAFTDSER 1472.66 1617.79 145.13 1 3,4
16 LDTGNFSWGSEAVTR 1638.76 1783.87 145.11 1 3,4
17 LWQVPETLPAEVVGK 1664.93 1954.13 289.19 2 3,4
18 QLDAHIEEQFGGGR 1555.73 1700.85 145.11 2 3,4
19 GFGFVTFAEEK 1230.61 1519.80 289.19 2 3,4
20 AFLVEEQK 962.51 1251.72 289.21 2 3,4
21 IFEGEALLR 1046.59 1191.69 145.10 2 3,4
22 ISGLIYEETR 1179.61 1324.72 145.11 2 3,4
23 TTAEEGVVALWR 1330.71 1475.80 145.09 2 3,4
24 LLIQNQDEMIK 1343.72 1632.92 289.21 2 3,4
25 IQDKEGIPPDQQR 1522.77 1811.99 289.22 2 3,4
26 TMVYPEAGFELQR 1539.74 1684.85 145.10 2 3,4
27 EQDVSLGANKFPER 1588.77 1878.00 289.22 2 3,4
28 GQGGIQQLLAAEQEAQR 1795.93 1941.03 145.10 2 3,4
29 QYAVFDEK$ 981.46 1287.68 306.22 2 3,4
30 QLDSHIEEQFGGGR$ 1554.71 1716.84 162.13 2 3,4
31 FDVGVKEIEGWTAR 1605.84 1895.03 289.19 2 3,4
32 HFEVDLSAFR 1219.61 1364.71 145.10 3 4
33 CALVYGQMNEPPGAR 1661.78 1806.87 145.10 4 6
34 NAVVTVPAYFNDSQR 1679.84 1824.94 145.10 4 5,6
35 QPTELELAQAFHQGK 1695.85 1985.07 289.22 4 5
36 TALTYVDNNDGSWHR 1747.80 1892.90 145.10 4 5
37 QQFPLALYQVDK 1448.78 1737.98 289.20 4 5,6
38 ADGFAGVFPEHK 1273.60 1562.82 289.22 4 5
39 SLEGLQANVQR 1213.63 1358.75 145.13 4 6
40 TFDNVYYK 1048.47 1337.70 289.23 4 5
41 MLSPLILGDEHYQTAR 1842.96 1988.04 145.08 4 5
42 SSFDAFQQILK 1282.67 1571.87 289.20 4 5
43 SSDFLMYGIK 1159.57 1448.77 289.20 4 5
44 SSMDAFQQILK 1282.67 1571.83 289.17 5 5
45 FTQANSEVSALLGR 1491.77 1636.88 145.11 5 6
46 STLVWEVR 988.56 1133.64 145.08 5 6
47 IFLENVIR 1002.62 1147.70 145.07 5 6
48 FFCEFCGK 1093.48 1382.65 289.17 5 6
49 EVAGFAPYEKR 1265.65 1554.85 289.20 5,6 6
50 SFGPAVIFNNEK 1321.70 1610.88 289.18 5 6
51 VALINYGPEYGR 1350.75 1495.80 145.06 5 6
52 YIAPEQVPVK 1142.63 1431.85 289.21 5 5,6
53 VEPLVNMGQITR 1355.72 1500.83 145.12 5 6
54 AYEPILLLGR 1143.67 1288.77 145.10 5 6
55 LVGEYGLR 905.51 1050.61 145.10 5 6
56 EALGGLPLYQR 1215.66 1360.77 145.11 5 6
57 ADAFLLVGTQPR 1286.70 1431.81 145.10 5 6,7
58 HGWEYVVK 1016.51 1305.72 289.21 6 8
59 FVIGGPHGDAGLTGR 1452.75 1597.86 145.10 7 8
60 LVNVFTIGK 989.60 1278.80 289.20 7 9,10
61 THAVVEPFVIATNR 1552.86 1697.95 145.09 7 8,9
62 SVHEPMQTGLK 1225.62 1514.82 289.21 7 8
63 SVVYALSPFQQK 1365.74 1654.94 289.20 7 10
64 YGGGANFVHDGYNK 1497.61 1786.88 289.27 7 8
65 TALTYIDGNGNWHR 1616.76 1761.88 145.12 8 9
66 AHLQDYIQTHYTAPR 1812.89 1958.00 145.11 8 9
67 NYTNAFQALYR 1359.66 1504.77 145.11 10 12
68 TLHPNWSPAAIK 1333.72 1622.93 289.21 10 11
69 FHQYQVVGR 1132.56 1277.69 145.13 11 12
70 FQSLGVAFYR 1186.64 1331.72 145.08 11 12
  1. This table presents 70 peptides shifted to more basic OGE fraction after iTRAQ labelling. The non-labelled (NL) and iTRAQ labelled (L) peptide masses are shown in this table together with the mass difference induced by the labelling. The OGE fractions (F) of iTRAQ labelled peptides and their native counterparts are as well presented.