Table 1 Observed basic pI shifts in OGE fractionation after iTRAQ labelling
Sequence | Mass NL | Mass L | Difference | F NL | F L | |
|---|---|---|---|---|---|---|
1 | ECADLWPR | 1045.46 | 1190.57 | 145.11 | 1 | 3,4 |
2 | AYLEDFYR | 1075.50 | 1220.61 | 145.11 | 1 | 3,4 |
3 | EQMGYTFDALK | 1301.58 | 1590.81 | 289.23 | 1 | 3,4 |
4 | TMADEGVVALWR | 1346.65 | 1491.77 | 145.12 | 1,2 | 3,4 |
5 | ECSGVEPQLWAR | 1430.66 | 1575.77 | 145.11 | 1 | 3,4 |
6 | NQIDEIVLVGGSTR | 1499.78 | 1644.90 | 145.13 | 1 | 3,4 |
7 | LAEMPADSGYPAYLAAR | 1794.84 | 1939.97 | 145.13 | 1 | 3,4 |
8 | ELEFYMK | 958.46 | 1247.66 | 289.20 | 1 | 3 |
9 | IPSAVGYQPTLSTDLGGLQER | 2201.13 | 2346.24 | 145.11 | 1 | 3,4 |
10 | AQIWDTAGQER | 1273.59 | 1418.71 | 145.12 | 1 | 3,4 |
11 | AGGECLTFDQLALR | 1549.77 | 1694.87 | 145.10 | 1 | 3,4 |
12 | VDFAYSFFEK | 1251.61 | 1540.79 | 289.18 | 1 | 3 |
13 | IFDKPEDFIAER | 1478.74 | 1767.95 | 289.22 | 1 | 3,4 |
14 | GLFTSDQILFTDTR | 1612.79 | 1757.92 | 145.13 | 1 | 3,4 |
15 | TTPSYVAFTDSER | 1472.66 | 1617.79 | 145.13 | 1 | 3,4 |
16 | LDTGNFSWGSEAVTR | 1638.76 | 1783.87 | 145.11 | 1 | 3,4 |
17 | LWQVPETLPAEVVGK | 1664.93 | 1954.13 | 289.19 | 2 | 3,4 |
18 | QLDAHIEEQFGGGR | 1555.73 | 1700.85 | 145.11 | 2 | 3,4 |
19 | GFGFVTFAEEK | 1230.61 | 1519.80 | 289.19 | 2 | 3,4 |
20 | AFLVEEQK | 962.51 | 1251.72 | 289.21 | 2 | 3,4 |
21 | IFEGEALLR | 1046.59 | 1191.69 | 145.10 | 2 | 3,4 |
22 | ISGLIYEETR | 1179.61 | 1324.72 | 145.11 | 2 | 3,4 |
23 | TTAEEGVVALWR | 1330.71 | 1475.80 | 145.09 | 2 | 3,4 |
24 | LLIQNQDEMIK | 1343.72 | 1632.92 | 289.21 | 2 | 3,4 |
25 | IQDKEGIPPDQQR | 1522.77 | 1811.99 | 289.22 | 2 | 3,4 |
26 | TMVYPEAGFELQR | 1539.74 | 1684.85 | 145.10 | 2 | 3,4 |
27 | EQDVSLGANKFPER | 1588.77 | 1878.00 | 289.22 | 2 | 3,4 |
28 | GQGGIQQLLAAEQEAQR | 1795.93 | 1941.03 | 145.10 | 2 | 3,4 |
29 | QYAVFDEK$ | 981.46 | 1287.68 | 306.22 | 2 | 3,4 |
30 | QLDSHIEEQFGGGR$ | 1554.71 | 1716.84 | 162.13 | 2 | 3,4 |
31 | FDVGVKEIEGWTAR | 1605.84 | 1895.03 | 289.19 | 2 | 3,4 |
32 | HFEVDLSAFR | 1219.61 | 1364.71 | 145.10 | 3 | 4 |
33 | CALVYGQMNEPPGAR | 1661.78 | 1806.87 | 145.10 | 4 | 6 |
34 | NAVVTVPAYFNDSQR | 1679.84 | 1824.94 | 145.10 | 4 | 5,6 |
35 | QPTELELAQAFHQGK | 1695.85 | 1985.07 | 289.22 | 4 | 5 |
36 | TALTYVDNNDGSWHR | 1747.80 | 1892.90 | 145.10 | 4 | 5 |
37 | QQFPLALYQVDK | 1448.78 | 1737.98 | 289.20 | 4 | 5,6 |
38 | ADGFAGVFPEHK | 1273.60 | 1562.82 | 289.22 | 4 | 5 |
39 | SLEGLQANVQR | 1213.63 | 1358.75 | 145.13 | 4 | 6 |
40 | TFDNVYYK | 1048.47 | 1337.70 | 289.23 | 4 | 5 |
41 | MLSPLILGDEHYQTAR | 1842.96 | 1988.04 | 145.08 | 4 | 5 |
42 | SSFDAFQQILK | 1282.67 | 1571.87 | 289.20 | 4 | 5 |
43 | SSDFLMYGIK | 1159.57 | 1448.77 | 289.20 | 4 | 5 |
44 | SSMDAFQQILK | 1282.67 | 1571.83 | 289.17 | 5 | 5 |
45 | FTQANSEVSALLGR | 1491.77 | 1636.88 | 145.11 | 5 | 6 |
46 | STLVWEVR | 988.56 | 1133.64 | 145.08 | 5 | 6 |
47 | IFLENVIR | 1002.62 | 1147.70 | 145.07 | 5 | 6 |
48 | FFCEFCGK | 1093.48 | 1382.65 | 289.17 | 5 | 6 |
49 | EVAGFAPYEKR | 1265.65 | 1554.85 | 289.20 | 5,6 | 6 |
50 | SFGPAVIFNNEK | 1321.70 | 1610.88 | 289.18 | 5 | 6 |
51 | VALINYGPEYGR | 1350.75 | 1495.80 | 145.06 | 5 | 6 |
52 | YIAPEQVPVK | 1142.63 | 1431.85 | 289.21 | 5 | 5,6 |
53 | VEPLVNMGQITR | 1355.72 | 1500.83 | 145.12 | 5 | 6 |
54 | AYEPILLLGR | 1143.67 | 1288.77 | 145.10 | 5 | 6 |
55 | LVGEYGLR | 905.51 | 1050.61 | 145.10 | 5 | 6 |
56 | EALGGLPLYQR | 1215.66 | 1360.77 | 145.11 | 5 | 6 |
57 | ADAFLLVGTQPR | 1286.70 | 1431.81 | 145.10 | 5 | 6,7 |
58 | HGWEYVVK | 1016.51 | 1305.72 | 289.21 | 6 | 8 |
59 | FVIGGPHGDAGLTGR | 1452.75 | 1597.86 | 145.10 | 7 | 8 |
60 | LVNVFTIGK | 989.60 | 1278.80 | 289.20 | 7 | 9,10 |
61 | THAVVEPFVIATNR | 1552.86 | 1697.95 | 145.09 | 7 | 8,9 |
62 | SVHEPMQTGLK | 1225.62 | 1514.82 | 289.21 | 7 | 8 |
63 | SVVYALSPFQQK | 1365.74 | 1654.94 | 289.20 | 7 | 10 |
64 | YGGGANFVHDGYNK | 1497.61 | 1786.88 | 289.27 | 7 | 8 |
65 | TALTYIDGNGNWHR | 1616.76 | 1761.88 | 145.12 | 8 | 9 |
66 | AHLQDYIQTHYTAPR | 1812.89 | 1958.00 | 145.11 | 8 | 9 |
67 | NYTNAFQALYR | 1359.66 | 1504.77 | 145.11 | 10 | 12 |
68 | TLHPNWSPAAIK | 1333.72 | 1622.93 | 289.21 | 10 | 11 |
69 | FHQYQVVGR | 1132.56 | 1277.69 | 145.13 | 11 | 12 |
70 | FQSLGVAFYR | 1186.64 | 1331.72 | 145.08 | 11 | 12 |