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Figure 4 | Proteome Science

Figure 4

From: Novel immune-modulator identified by a rapid, functional screen of the parapoxvirus ovis (Orf virus) genome

Figure 4

The B2 protein directs accumulation of immune cells and enhances an antigen-specific antibody response. A. Mice were injected i.d. in the abdomen with 50 ng of the irrelevant recombinant (r)SRB4 protein (rCTRL) (left panel) or recombinant (r)B2 protein (right panel); the 2 proteins were injected into separate abdominal sites of 3 mice. Both proteins were produced in E. coli by the same protocol and identically purified. After 4 days, immune cell densities were measured in harvested skin by 2G9 immuno-staining, as described in Figures 2 and 3. Scale bars are 40 micron. B. Mice were co-injected i.d. in the abdomen with 1 μg rHBsAg alone (▲), 1 μg rHBsAg with 50 ng of rB2 protein (), or 1 μg rHBsAg with 50 ng of the rSRB4 control protein (rCTRL) (O). Sera collected over a 2 month time-course were assayed for antibodies to HBsAg by ELISA, with an HRP-readout as described in Material and Methods. No boosts were administered. Antibody levels of experimental samples were calculated relative to absorbance (A450) values measured for known amounts of a commercial anti-HBsAg antibody (Seradyn, Inc). Calculated antibody levels are plotted; error bars are SDs from the arithmetic mean. Student's t-test was used to compare antibody levels at day 56.

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