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Figure 5 | Proteome Science

Figure 5

From: Novel immune-modulator identified by a rapid, functional screen of the parapoxvirus ovis (Orf virus) genome

Figure 5

Protective immunity against influenza virus conferred by a hemagglutinin gene vaccine is enhanced by co-administration of the B2L gene. Groups of BALB/c mice were immunized with a series of pCMVi-HA (pHA) doses: 0.04 ng, 4 ng, 40 ng, and 400 ng, as indicated above each graph, and represent total mouse doses which were delivered in 2 gene gun discharges (shots). This gene vaccine plasmid (pHA) was co-delivered with 1 μg pUC119 as a negative control plasmid (pCTRL) (short dashes), 1 μg pCMVi-B2L (pB2L) (solid line), or 1 μg pCMVi-GMCSF (pGMCSF) as a positive control plasmid (long dashes), delivered in 2 shots. Animals were given a second immunization with the same inocula and dose 3 weeks post-prime. Mice were challenged with a lethal intranasal dose of influenza A virus 6 weeks after the boost. All mice in the control, unimmunized group died on or before day 12. The Kaplan-Meir survival curves for each group of mice (8 mice per group) are displayed. A parametric survival test (Prism 4, Graphpad, Inc.) was used to measure significant differences between survival rates observed at the 400 ng HA gene vaccine dose.

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