Skip to main content

Table 3 Primer sequences used in RT-PCR

From: Proteomic analysis of the effects of exogenous calcium on hypoxic-responsive proteins in cucumber roots

Transcripts

Product length (bp)

Annealing temp. (°C)

Primer pairs

ahd

714

57

S 5′-TCAAGGTCGCCAATCCCA-3′

   

AS 5′-TATGCCAGCAGCCTCAAAC-3′

vatps

427

57

S 5′-GGCAGTGTTACTATTGTCGG-3′

   

AS 5′-TATTACGCATCATCCAGACC-3′

adh

415

57

S 5′-AGGGTTCATCTGTTGCTATCT-3′

   

AS 5′-GGAATGTCAGTTCTCGGTTT-3′

ela

455

55

S 5′-GTGGATTCGCTCCTAACA-3′

   

AS 5′-TTTCACAGCCTCAATACTCT-3′

ss

318

57

S 5′-GAGTTTGAGGTCCAGACTATTT-3′

   

AS 5′-AATTTAACTGCTGCCTGATT-3′

gsk

663

53

S 5′-TGCCAGTTGGACTTGACG-3′

   

AS 5′-GGGATGCTCGCTGATACA-3′

gs

427

55

S 5′-CCAGGAGAAGACAGTGAA-3′

   

AS 5′-AGATGTAACGAGCAACCC-3′

pdh

437

55

S 5′-CCCCAAATCTACCGTCTC-3′

   

AS 5′-AACTCCACAACAGGCTTC-3′

mdh

294

53

S 5′-TGAATGGCGTAAAGATGG-3′

   

AS 5′-GGATGGAAGGAGCAAACT-3

pod

463

57

S 5′-ATTCGCCTCCATTTCCAT-3′

   

AS 5′-GGCTTCCAGTTCCGTTGA-3′

atpsβ

459

57

S 5′-TTGACCAGGCAACGGAACA-3′

   

AS 5′-TACGACCAAGCAAAGCAGACAC-3′

ald

546

57

S 5′-CTACAGAGGCAAATACGCT-3′

   

AS 5′-TCAGGCTCCACAATAGGT-3′

actin

290

58

S 5′-CCGTTCTGTCCCTCTACGCTAGTG-3′

   

AS 5′-GGAACTGCTCTTTGCAGTCTCGAG-3

  1. S: Sense primer; AS: Anti-sense primer.