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Figure 1 | Proteome Science

Figure 1

From: The role of nitric oxide on rosuvastatin-mediated S-nitrosylation and translational proteomes in human umbilical vein endothelial cells

Figure 1

Rosuvastatin increases eNOS activation and subsequent protein S-nitrosylation in ECs. (A) Protein lysates (40 μg) extracted from ECs treated with rosuvastatin (0.1 μM, 1 μM and 10 μM) for 24 h, or (B) rosuvastatin (10 μM) for 0.5, 6, and 24 h were separated by SDS-PAGE and western blotting with antibodies against eNOS (1:3000), phospho-eNOSS1177 (peNOSS1177, 1:3000) and β-actin (1:5000). The relative fold eNOS and peNOSS1177 are shown by mean ± S.E. compared with control treatment. # p < 0.05, *p < 0.01 from three separate experiments by using Fisher’s LSD. (C) One milligram of biotin-switch-derived biotinylated lysate collected from ECs with rosuvastatin (10 μM) treatment for various intervals (0.5, 6 and 24 h) was analyzed by western blot-based 2-DE (streptavidin-HRP, 1:3000). The spot numbers detected on the 2-DE x-ray films are shown as mean ± S.E. from three independent experiments.

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