Figure 3

Rosuvastatin-induced protein S-nitrosylation is a posttranslational event. (A) The 2-DE gels were stained by VisPRO dye to evaluate translational levels. Western blotting was subsequently performed to determine posttranslational levels. Open triangles indicate reference proteins in each gel and x-ray film for confirming the accurate position of each protein spot. Protein spots with increased S-nitrosylation are indicated by arrowheads. (B) Rosuvastatin-induced changes at the translational level (column labeled “VisPRO”) and posttranslational S-nitrosylation (column labeled “western blot”) of each S-nitrosoprotein represented as relative fold changes (black bar/gray bar). Statistical data are shown as mean ± S.E. from three separate experiments.