The role of nitric oxide on rosuvastatin-mediated S-nitrosylation and translational proteomes in human umbilical vein endothelial cells

Table 1 S-nitrosylated proteins identified by LC-MS/MS

Spot number	Protein name ^a)	Accession number ^b)	Theoretical MW (kDa)/pI ^c)	Experimental MW (kDa)/pI ^d)	Sequence coverage (%)	MOWSE score	Peptides matched
ES1	HSP70	http://21040386	73.8/6.0	76.3/5.8	47	670	24
ES2	HSP70	http://21040386	73.8/6.0	75.8/5.8	50	717	29
ES3	ER-60 protease	1208427	57.2/5.9	82.5/4.6	26	442	8
ES4	HSP60	31542947	61.0/5.7	63.4/5.6	24	337	10
ES5	mt-HSP70 ^b)	14917005	59.2/5.9	57.2/5.8	34	257	12
ES6	Vimentin	http://340219	53.6/5.0	56.4/4.8	65	998	26
ES7	Vimentin	http://340219	53.6/5.0	56.2/4.7	49	798	21
ES8	Vimentin	http://340219	53.6/5.0	55.8/4.6	55	824	23
ES9	Tubulin	34740335	50.1/4.9	55.0/4.6	32	367	11
ES10	PDI-related protein	1710248	46.2/5.0	56.3/5.0	20	284	7
ES11	PDI A3 precursor	729433	56.9/6.2	55.4/6.4	37	571	11
ES12	Beta-actin	4501885	41.7/5.3	57.2/5.2	61	872	21
ES13	Beta-actin	4501885	41.7/5.3	56.4/5.1	42	605	14
ES14	Phospholipase C-alpha	303618	57.1/6.2	64.2/5.7	11	194	3
ES15	Transaldolase	5803187	37.7/6.4	54.8/5.6	16	269	6
ES16	Tropomyosin 3	55665775	29.1/4.8	34.5/4.7	27	371	6
ES17	Tropomyosin 3	55665775	29.1/4.8	28.6/4.6	28	395	7

a) The function of the protein was obtained via the MASCOT software (www.matrixscience.com) search program by querying the NCBInr database. The parameters were set as follows: peptide mass tolerance: ± 0.4 Da; allowed missed cleavage: 1.
b) Accession number from the NCBInr database.
c) Protein molecular weight and pI annotated in the database.
d) Protein molecular weight and pI calculated from 2-DE gels.

ISSN: 1477-5956