Sex differences in the response of the alveolar macrophage proteome to treatment with exogenous surfactant protein-A

Table 1 Significant changes in alveolar macrophage proteins for Female mice (as compared to KO baseline)

Gel no.	Protein name	KO 6 hr SP-A Female	KO 18 hr SP-A Female	WT Female
5	Alpha-fetoprotein	↑*	↑	↑
8	Anxa5 protein	↑	↓	↑*
11	Calpain, small subunit 1	↑	↓*	↓
12	Capping protein (actin filament) muscle Z-line, alpha 2 (CapZ alpha-2)	↓*	↓*	↓
13	Capping protein (actin filament) muscle Z-line, beta isoform a (CapZ beta)	↓*	↓	↓
24	Eno1 protein (Alpha-enolase)	↑*	↑	↑
25	Eukaryotic translation initiation factor 5A	↑	↑*	↑
26	Ezrin	↓*	↓	↓
29	Ferritin light chain 1	↓	↓	↓*
30	Gamma-actin	↓	↓*	↓
34	Heat shock protein 1, beta (HSP90AB1)	↓*	↓	↓*
35	Heat shock protein 5 precursor (GRP78)	↑	↑	↓*
40	Heme-binding protein	↓*	↓	↓*
41	Heterogeneous nuclear ribonucleoprotein K	↓*	↓	↓
45	Keratin complex 2, basic, gene 8	↓	↑	↑*
52	Nucleophosmin 1	↓	↓*	↓*
53	p50b	↓*	↓	↓
55	Prolyl 4-hydroxylase, beta polypeptide precursor	↑*	↑*	↓
56	Proteasome (prosome, macropain) 28 subunit, alpha	↑*	↑	↑*
61	Purine nucleoside phosphorylase	↑*	↑	↑*
64	Rho GDP dissociation inhibitor (GDI) alpha	↑	↑*	↑*
67	Stathmin	↑	↑*	↑*
74	Vacuolar adenosine triphosphatase subunit B	↑*	↑	↑
76	Vimentin	↓	↓*	↓
	Total significant changes	7↓, 6↑	5↓, 4↑	5↓, 6↑

Comparison of mean normalized volumes (see Additional file 3) for proteins from female KO mice to KO 6 hr SP-A, KO 18 hr SP-A, and WT mice. Increased compared to KO(↑), decreased compared to KO(↓), determined to be significant (p < 0.05) by t-test (*). Changes in all proteins are given in Additional file 4 - Table F.

ISSN: 1477-5956