Sex differences in the response of the alveolar macrophage proteome to treatment with exogenous surfactant protein-A

Table 2 Significant changes in alveolar macrophage proteins from male to female mice in the same group

Gel no.	Protein name	KO	KO 6 hr SP-A	KO 18 hr SP-A	WT
6	Annexin A2	↓*	↓	↓	UN
7	Annexin A4	↓	↑	↓*	↓
8	Anxa5 protein	↓	↓	↓*	↓
12	Capping protein (actin filament) muscle Z-line, alpha 2 (CapZ alpha-2)	↓	↓	↓*	↓
15	Chaperonin subunit 2 (beta) (CCT2)	↑	↑	↑	↑*
17	Chitinase 3-like 3 precursor (Ym1)	UN	↑	↑*	↑*
20	Chloride intracellular channel 4 (mitochondrial)	↓	↑	↓*	↓
22	Coactosin-like 1	↓	↓	↓*	↓
33	Guanine deaminase	↑*	↑*	↑*	↑*
34	Heat shock protein 1, beta (HSP90AB1)	↑*	↑	↑*	↑
35	Heat shock protein 5 precursor (GRP78)	↑*	↑	↑*	↑
36	Heat shock protein 65 (HSP60)	↑	↑	↑*	↑*
38	Heat shock protein 90, beta (Grp94), member 1	↓	↑	↑*	↑
40	Heme-binding protein	UN	↓*	UN	↓
49	Major vault protein (MVP)	↑	↑	↑*	↑*
50	Microtubule-associated protein, RP/EB family, member 1	↓	↓	↑*	↑*
55	Prolyl 4-hydroxylase, beta polypeptide precursor	↓*	↓	↓	↓
59	Protein disulfide-isomerase A3 precursor	↑	↑*	↑	↑
60	Protein synthesis initiation factor 4A	↓	↑	↑*	↑*
62	Put. beta-actin (aa 27–375)	↓	↓	↓*	↓
63	Rab GDP dissociation inhibitor beta	↓	↑	↑	↑*
64	Rho GDP dissociation inhibitor (GDI) alpha	↓	↑	↑*	↑*
69	Tropomodulin 3	↓	↓*	↓*	↓
70	Tropomyosin 3, gamma	↓	↓	↓*	↓
71	Tubulin, beta 5	↓	↓*	↓	↓*
75	Valosin-containing protein	↑	↑	↑*	↑*
76	Vimentin	↓	↓*	↓*	↓
	Total significant changes	2↓, 3↑	4↓, 2↑	9↓, 11↑	1↓, 10↑

Comparison of mean normalized volumes (see Additional file 3) for proteins from male to female mice in the same group (KO, KO 6 hr SP-A, KO 18 hr SP-A, and WT). Higher in female (↑), lower in female (↓), unchanged (UN), determined to be significant (p < 0.05) by t-test (*). Changes in all proteins are given in Additional file 5 -Table F.

ISSN: 1477-5956