Figure 6

Validation of S100A6 in cytoplasmic extract of AD-MSC. A) SELDI-ToF immunoassay of m/z 10,087. Cytoplasmic extract from a pool of AD-MSC at passage 7: 1) spotted on CM10 array; 2) spotted on PS10 array indirectly coupled to anti-S100A6 antibody; 3) eluted from PS10 array indirectly coupled to anti-S100A6 antibody and spotted on CM10 array. The signals enclosed by the rectangle represent the peak at m/z 10,087. B) SDS-PAGE/Western blot analysis using anti-S100A6 and anti-Actin antibody of AD-MSC during different passages of cell expansion for different donors. C) Western blot densitometry quantification of the corresponding S100A6. Values are expressed as relative intensity ratio between S100A6 and Actin. D) Human S100-A6 ELISA quantification. Values are expressed as absolute quantification (μg/ml S100A6).