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Table 2 Discriminatory protein peaks identified in the discovery phase and confirmed in the validation phase

From: Identification of serum proteomic biomarkers for early porcine reproductive and respiratory syndrome (PRRS) infection

Fraction

Surface

Focus mass

ROC (regulation)

M/Z (kDalton)

p-value discovery

p-value validation

Sensitivity (+/+)

Specificity (−/−)

1

CM10

Low

0.69 (up-regulated)

4.151

0.02

0.00

  

1

CM10

Low

0.82 (up-regulated)

4.458

0.00

0.00

  

1

CM10

Low

0.32 (down-regulated)

5.468

0.04

0.01

  

1

CM10

Low

0.28 (down-regulated)

5.536

0.01

0.01

  

1

CM10

Low

0.74 (up-regulated)

8.308

0.00

0.00

  

1

CM10

Low

0.71 (up-regulated)

8.516

0.02

0.00

  

1

CM10

Low

0.88 (up-regulated)

8.918

0.00

0.00

  

1

CM10

Low

0.87 (up-regulated)

9.124

0.00

0.00

  

1

CM10

Low

0.82 (up-regulated)

11.404

0.00

0.02

  

1

CM10

Low

0.76 (up-regulated)

11.613

0.00

0.02

  

1

CM10

Low

0.69 (up-regulated)

13.785

0.02

0.04

  

1

CM10

Low

0.80 (up-regulated)

17.218

0.00

0.00

  

1

CM10

Low

0.80 (up-regulated)

17.838

0.00

0.00

  

1

CM10

Low

0.74 (up-regulated)

19.761

0.00

0.02

  

Total number of significant peaks Fraction 1, CM10, low focus mass: 14

77%

73%

1

CM10

High

0.82 (up-regulated)

20.322

0.00

0.00

  

1

CM10

High

0.77 (up-regulated)

23.496

0.00

0.00

  

1

CM10

High

0.76 (up-regulated)

54.107

0.00

0.04

  

1

CM10

High

0.76 (up-regulated)

101.410

0.00

0.01

  

1

CM10

High

0.76 (up-regulated)

135.096

0.00

0.01

  

1

CM10

High

0.87 (up-regulated)

147.351

0.00

0.00

  

Total number of significant peaks Fraction 1, CM10, high focus mass: 6

58.8%

51.5%

1

IMAC30

Low

0.84 (up-regulated)

4.462

0.00

0.006

  

1

IMAC30

Low

0.80 (up-regulated)

8.843

0.00

0.011

  

1

IMAC30

Low

0.84 (up-regulated)

8.914

0.00

0.016

  

1

IMAC30

Low

0.77 (up-regulated)

8.977

0.001

0.008

  

1

IMAC30

Low

0.82 (up-regulated)

9.119

0.00

0.009

  

1

IMAC30

Low

0.79 (up-regulated)

9.136

0.00

0.006

  

1

IMAC30

Low

0.64 (up-regulated)

11.090

0.056

0.04

  

1

IMAC30

Low

0.79 (up-regulated)

17.860

0.00

0.013

  

Total number of significant peaks Fraction 1, IMAC30, low focus mass: 8

60.6%

51.5%

4

CM10

High

0.70 (up-regulated)

23.162

0.02

0.00

  

4

CM10

High

0.67 (up-regulated)

89.049

0.02

0.017

  

Total number of significant peaks Fraction 4, CM10, high focus mass: 2

  

4

IMAC30

High

0.67 (up-regulated)

144.495

0.034

0.00

  

Total number of significant peaks Fraction 4, IMAC30, high focus mass: 1

  

6

CM10

Low

0.76 (up-regulated)

4.161

0.008

0.00

  

6

CM10

Low

0.70 (up-regulated)

8.328

0.025

0.013

  

6

CM10

Low

0.68 (up-regulated)

8.535

0.041

0.008

  

6

CM10

Low

0.70 (up-regulated)

8.552

0.029

0.010

  

6

CM10

Low

0.70 (up-regulated)

8.642

0.036

0.013

  

6

CM10

Low

0.30 (down-regulated)

14.843

0.010

0.015

  

Total number of significant peaks Fraction 6, CM10, low focus mass: 6

64.5%

69.7%

6

IMAC30

Low

0.74 (up-regulated)

8.928

0.013

0.029

  

6

IMAC30

Low

0.70 (up-regulated)

10.041

0.025

0.025

  

6

IMAC30

Low

0.76 (up-regulated)

11.412

0.005

0.00

  

6

IMAC30

Low

0.74 (up-regulated)

12.237

0.009

0.002

  

6

IMAC30

Low

0.74 (up-regulated)

12.522

0.009

0.004

  

6

IMAC30

Low

0.76 (up-regulated)

12.930

0.002

0.003

  

6

IMAC30

Low

0.78 (up-regulated)

13.143

0.002

0.004

  

6

IMAC30

Low

0.68 (up-regulated)

17.171

0.045

0.018

  

Total number of significant peaks Fraction 6, IMAC30, low focus mass: 8

54.5%

53%

6

IMAC30

High

0.28 (down-regulated)

27.806

0.023

0.018

  

6

IMAC30

High

0.30 (down-regulated)

27.606

0.030

0.017

  

Total number of significant peaks Fraction 6, IMAC30, high focus mass: 2

  
  1. Proteomic features of the 47 discriminatory protein peaks identified by SELDI-TOF MS in the discovery phase and confirmed in the validation phase. The peaks are divided by fraction, array surface, acquisition focus mass (low: 1–20 kDa; high: 20–200 kDa), ROC (Receiver Operating Characteristic = Area Under Curve) value with regulation status in PRRSV-positive compared to PRRSV-negative piglets, molecular weight, and p-values for both discovery and validation phases. The sensitivity and specificity of the total number of discriminatory peaks identified per fraction, array surface and acquisition focus mass is also reported. The sensitivity and specificity were calculated only if the number of peaks was greater than 2.
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