From: Proteomic analysis of ‘Zaosu’ pear (Pyrus bretschneideri Rehd.) and its red skin bud mutation
Spot no | Protein name | Accession no. | Protein MW/PI | Pep. count | Proten score | Ratio ‘Zaosu’/the red mutant | Relative protein content |
---|---|---|---|---|---|---|---|
Photosynthetic and energy metabolism proteins | |||||||
D6 | ribìlose-1,5-bisphosphate carboxylase/ oxygenase large subunit | gi|15148414 | 52493.4/6.32 | 21 | 702 | 2.57 |
|
D7 | ribìlose-1,5-bisphosphate carboxylase/ oxygenase large subunit Precursor | gi|7008057 | 53067.6/6.04 | 14 | 520 | 1.62 |
|
D9 | LHCII type I chlorophyll a/b binding protein | gi|8954293 | 27900.1/5.29 | 3 | 102 | 0.47 |
|
D13 | Ribìlose bisphosphate carboxylase/ oxygenase activase | gi|10720248 | 48341.7/8.19 | 7 | 322 | 5.97 |
|
D17 | oxygen-evolving complex protein 1 | gi|739292 | 26603.5/5.13 | 11 | 576 | 0.42 |
|
D18 | NAD-dependent malate dehydrogenase | gi|15982948 | 35817.5/6.6 | 8 | 255 | 0.49 |
|
E1 | ribìlose1,5-bisphosphate carboxylase | gi|9909955 | 52296.2/6.04 | 10 | 305 | 2.60 |
|
Stress-related proteins | |||||||
D4 | Heat shock protein 70 [Cucumis sativus] | gi|1143427 | 75480/5.15 | 23 | 850 | 0.51 |
|
D14 | shock 70 kDa protein, mitochondrial | gi|399940 | 72720.6/5.95 | 12 | 98 | 0.37 |
|
D19 | high molecìlar weight heat shock protein | gi|6969976 | 71570.4/5.17 | 24 | 743 | 0.37 |
|
E2 | Heat shock cognate 70 kDa protein 2 | gi|123620 | 71062/5.08 | 22 | 555 | 3.47 |
|
Disease resistance-related proteins | |||||||
D16 | Pbzs315 SSH cDNA library of pear cv. ‘Zaosu’ inocìlated with Venturia nashicola Pyrus x bretschneideri cDNA, mRNA sequence | gi|255987829 | 35747.7/9.88 | 6 | 279 | 0.33 |
|
D20 | polyphenol oxidase precursor | gi|3282505 | 67432.9/6.39 | 4 | 273 | 0.47 |
|
D21 | S-adenosysl-L-methionine:caffeic acid3-O-methyltransferase | gi|3913295 | 40135.3/5.52 | 7 | 247 | 0.38 |
|
D24 | polyphenol oxidase 2 precursor | gi|14194273 | 65762/6.09 | 7 | 178 | 1.72 |
|
Cytoskeleton-related proteins | |||||||
D15 | Os03g0718100 | gi|115454971 | 42014/5.3 | 14 | 255 | Only observed in the red mutant |
|
D22 | actin | gi|32186902 | 42057.1/5.3 | 16 | 531 | Only observed in the red mutant |
|
Amino acid metabolism-related proteins | |||||||
D23 | S-adenosylmethionine synthase | gi|75308025 | 43564.9/5.5 | 14 | 635 | 0.53 |
|
E4 | putative methionine synthase | gi|14532772 | 84899.8/6.09 | 12 | 148 | Only observed in ‘Zaosu’ pear |
|
Antioxidant-related proteins | |||||||
D11 | Putative pyridoxine biosynthesis protein isoform A | gi|46399269 | 33353.1/5.93 | 8 | 218 | 0.24 |
|
Calcium-related proteins | |||||||
D10 | Calreticìlin | gi|11131904 | 48557.5/4.4 | 14 | 380 | 0.49 |
|
Others | |||||||
E3 | Ipa. ADAB-aaa60g07.b1 IPA1-seed Arachis ipaensis cDNA 5', mRNA sequence | gi|296593933 | 23794.4/9.29 | 11 | 92 | 1.60 |
|