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Table 1 Proteins result analyzed and identified by MALDI-TOF-TOF/MS

From: Proteomic analysis of ‘Zaosu’ pear (Pyrus bretschneideri Rehd.) and its red skin bud mutation

Spot no

Protein name

Accession no.

Protein MW/PI

Pep. count

Proten score

Ratio ‘Zaosu’/the red mutant

Relative protein content

Photosynthetic and energy metabolism proteins

 

  D6

ribìlose-1,5-bisphosphate carboxylase/ oxygenase large subunit

gi|15148414

52493.4/6.32

21

702

2.57

  D7

ribìlose-1,5-bisphosphate carboxylase/ oxygenase large subunit Precursor

gi|7008057

53067.6/6.04

14

520

1.62

  D9

LHCII type I chlorophyll a/b binding protein

gi|8954293

27900.1/5.29

3

102

0.47

  D13

Ribìlose bisphosphate carboxylase/ oxygenase activase

gi|10720248

48341.7/8.19

7

322

5.97

  D17

oxygen-evolving complex protein 1

gi|739292

26603.5/5.13

11

576

0.42

  D18

NAD-dependent malate dehydrogenase

gi|15982948

35817.5/6.6

8

255

0.49

  E1

ribìlose1,5-bisphosphate carboxylase

gi|9909955

52296.2/6.04

10

305

2.60

Stress-related proteins

  

  D4

Heat shock protein 70 [Cucumis sativus]

gi|1143427

75480/5.15

23

850

0.51

  D14

shock 70 kDa protein, mitochondrial

gi|399940

72720.6/5.95

12

98

0.37

  D19

high molecìlar weight heat shock protein

gi|6969976

71570.4/5.17

24

743

0.37

  E2

Heat shock cognate 70 kDa protein 2

gi|123620

71062/5.08

22

555

3.47

Disease resistance-related proteins

  

  D16

Pbzs315 SSH cDNA library of pear cv. ‘Zaosu’ inocìlated with Venturia nashicola Pyrus x bretschneideri cDNA, mRNA sequence

gi|255987829

35747.7/9.88

6

279

0.33

  D20

polyphenol oxidase precursor

gi|3282505

67432.9/6.39

4

273

0.47

  D21

S-adenosysl-L-methionine:caffeic acid3-O-methyltransferase

gi|3913295

40135.3/5.52

7

247

0.38

  D24

polyphenol oxidase 2 precursor

gi|14194273

65762/6.09

7

178

1.72

Cytoskeleton-related proteins

  

  D15

Os03g0718100

gi|115454971

42014/5.3

14

255

Only observed in the red mutant

  D22

actin

gi|32186902

42057.1/5.3

16

531

Only observed in the red mutant

Amino acid metabolism-related proteins

  

  D23

S-adenosylmethionine synthase

gi|75308025

43564.9/5.5

14

635

0.53

  E4

putative methionine synthase

gi|14532772

84899.8/6.09

12

148

Only observed in ‘Zaosu’ pear

Antioxidant-related proteins

  

  D11

Putative pyridoxine biosynthesis protein isoform A

gi|46399269

33353.1/5.93

8

218

0.24

Calcium-related proteins

  

  D10

Calreticìlin

gi|11131904

48557.5/4.4

14

380

0.49

Others

  

  E3

Ipa. ADAB-aaa60g07.b1 IPA1-seed Arachis ipaensis cDNA 5', mRNA sequence

gi|296593933

23794.4/9.29

11

92

1.60