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Table 1 PPP1R12B phosphopeptides and predicted potential kinases

From: Insulin-stimulated phosphorylation of protein phosphatase 1 regulatory subunit 12B revealed by HPLC-ESI-MS/MS

Start Stop Peptide sequence Phosphorylation site Predicted kinases*
28 38 GpSLTEQEPAER Ser29 CK2α, CK2α’, DMPK, PAK-1, PAK-2, PAK-3, PAK-4, PKAα, PKAβ, p70-S6K
   GSLpTEQEPAER Thr31 CK2α, CK2α’
54 76 VRFEDGAVFLAACpSSGDTDEVRK Ser67 CK2α, CK2α’
445 458 pTGSHNMLSEVANSR Thr445 DMPK, NEK2
501 511 KLNpSTSDIEEK Ser504 CK2α, CK2α’
   KLNSTpSDIEEK Ser506 CK2α, CK2α’
645 659 pSpTQGVTLTDLQEAER Ser645/Thr646 AIM1, PAK-1, PAK-2, PAK-3, PKAα, PKAβ, DMPK, Pim-2h/ DMPK, Pim-2h, RhoK
711 720 pSLDEEPICHR Ser711 CK2α, CK2α’
755 773 FSVPDpSESSETTTNTTTAK Ser760 CK2α, CK2α’
   FSVPDSEpSSETTTNTTTAK Ser762 CK2α, CK2α’, TLK1
837 853 LEpSGGSNPTTSDSYGDR Ser839 ActRIIA, ActRIIB, CLK1, CLK2, DMPK, RAGE-1, TGFR-2
   LESGGSNPTTpSDSYGDR Ser847 CK2α, CK2α’
   LESGGSNPTTSDpSYGDR Ser849 ActRIIA, ActRIIB, TGFR-2
945 954 KMpSEMEEEMK Ser947 CK2α, CK2α’
  1. * Using NetworKIN 2.0 Beta (http://networkin.info/version_2_0/newPrediction.php) as well as literature searches.
  2. Insulin-stimulated sites.
  3. Novel phosphorylation sites are indicated in boldface type.
  4. Kinase abbreviations: CK2α, casein kinase II, alpha chain; CK2α’, casein kinase II, alpha' chain; DMPK, myotonic dystrophy protein kinase; PAK-1, p21-activated kinase 1; PAK-2, p21-activated kinase 2; PAK-3, p21-activated kinase 3; PAK-4, p21-activated kinase 4; PKAα, cAMP-dependent protein kinase, alpha-catalytic subunit; PKAβ, cAMP-dependent protein kinase, beta-catalytic subunit; p70-S6K, ribosomal protein S6 kinase 1; NEK2, nimA-related protein kinase 2; AIM-1, Aurora- and Ipl1-like midbody-associated protein 1; Pim-2h, serine/threonine-protein kinase Pim-2; RhoK, Rho kinase, TLK1, tousled-like kinase 1; ACTRIIA, activin receptor type II precursor; ACTRIIB, activin receptor type IIB precursor; CLK1, CDC-like kinase 1; CLK2, CDC-like kinase 2; RAGE-1, renal tumor antigen 1; TGFR-2, TGF-beta receptor type II precursor.