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Table 1 PPP1R12B phosphopeptides and predicted potential kinases

From: Insulin-stimulated phosphorylation of protein phosphatase 1 regulatory subunit 12B revealed by HPLC-ESI-MS/MS

Start

Stop

Peptide sequence

Phosphorylation site

Predicted kinases*

28

38

GpSLTEQEPAER

Ser29

CK2α, CK2α’, DMPK, PAK-1, PAK-2, PAK-3, PAK-4, PKAα, PKAβ, p70-S6K

  

GSLpTEQEPAER

Thr31

CK2α, CK2α’

54

76

VRFEDGAVFLAACpSSGDTDEVRK

Ser67

CK2α, CK2α’

445

458

pTGSHNMLSEVANSR

Thr445

DMPK, NEK2

501

511

KLNpSTSDIEEK

Ser504

CK2α, CK2α’

  

KLNSTpSDIEEK

Ser506

CK2α, CK2α’

645

659

pSpTQGVTLTDLQEAER

Ser645/Thr646

AIM1, PAK-1, PAK-2, PAK-3, PKAα, PKAβ, DMPK, Pim-2h/ DMPK, Pim-2h, RhoK

711

720

pSLDEEPICHR

Ser711

CK2α, CK2α’

755

773

FSVPDpSESSETTTNTTTAK

Ser760

CK2α, CK2α’

  

FSVPDSEpSSETTTNTTTAK

Ser762

CK2α, CK2α’, TLK1

837

853

LEpSGGSNPTTSDSYGDR

Ser839

ActRIIA, ActRIIB, CLK1, CLK2, DMPK, RAGE-1, TGFR-2

  

LESGGSNPTTpSDSYGDR

Ser847

CK2α, CK2α’

  

LESGGSNPTTSDpSYGDR

Ser849

ActRIIA, ActRIIB, TGFR-2

945

954

KMpSEMEEEMK

Ser947

CK2α, CK2α’

  1. * Using NetworKIN 2.0 Beta (http://networkin.info/version_2_0/newPrediction.php) as well as literature searches.
  2. Insulin-stimulated sites.
  3. Novel phosphorylation sites are indicated in boldface type.
  4. Kinase abbreviations: CK2α, casein kinase II, alpha chain; CK2α’, casein kinase II, alpha' chain; DMPK, myotonic dystrophy protein kinase; PAK-1, p21-activated kinase 1; PAK-2, p21-activated kinase 2; PAK-3, p21-activated kinase 3; PAK-4, p21-activated kinase 4; PKAα, cAMP-dependent protein kinase, alpha-catalytic subunit; PKAβ, cAMP-dependent protein kinase, beta-catalytic subunit; p70-S6K, ribosomal protein S6 kinase 1; NEK2, nimA-related protein kinase 2; AIM-1, Aurora- and Ipl1-like midbody-associated protein 1; Pim-2h, serine/threonine-protein kinase Pim-2; RhoK, Rho kinase, TLK1, tousled-like kinase 1; ACTRIIA, activin receptor type II precursor; ACTRIIB, activin receptor type IIB precursor; CLK1, CDC-like kinase 1; CLK2, CDC-like kinase 2; RAGE-1, renal tumor antigen 1; TGFR-2, TGF-beta receptor type II precursor.