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Table 2 PPP1R12B internal standard peptides

From: Insulin-stimulated phosphorylation of protein phosphatase 1 regulatory subunit 12B revealed by HPLC-ESI-MS/MS

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Peptide sequence

Molecular weight (Da)

Quantified ( m/z )*

282

303

LGQTPFDVADEGLVEHLELLQK

2451.2766

817.7637 (+3)

    

1226.1419 (+2)

327

333

IQSGFFK

826.4458

413.7265 (+2)

    

826.4458 (+1)

397

417

SSITEQIPAPAQNTFSASSAR

2163.0677

721.6941 (+3)

    

1082.0375 (+2)

492

500

SYISSLAPR

993.5364

497.2718 (+2)

    

993.5364 (+1)

515

522

ESAVNLVR

887.4945

444.2509 (+2)

    

887.4945 (+1)

741

754

PSLYTSSHLLWTNR

1674.8598

558.9581 (+3)

    

837.9336 (+2)

837

853

LESGGSNPTTSDSYGDR

1742.7464

871.8768 (+2)

966

975

LKDENGALIR

1128.6371

564.8222 (+2)

    

1128.6371 (+1)

  1. * m/z, mass-to-charge ratio.
  2. Combined peak areas for the +1, +2, and +3 (when detected) charge states for each peptide were obtained by integration of the appropriate reconstructed ion chromatograms.