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Table 4 Effect of insulin on PPP1R12B phosphorylation, n = 4

From: Insulin-stimulated phosphorylation of protein phosphatase 1 regulatory subunit 12B revealed by HPLC-ESI-MS/MS

Ser29     
Experiment Normalized peak area # Ratio
  Control Insulin Control Insulin
  1 1.10E-05 3.89E-05 0.43 1.51
  2 1.20E-05 6.84E-05 0.46 2.65
  3 2.85E-05 5.64E-05 1.10 2.18
  4 5.18E-05 1.48E-04 2.01 5.75
Average 2.58E-05   1.00 3.02*
SEM    0.37 0.94
Ser504     
Experiment Normalized peak area # Ratio
  Control Insulin Control Insulin
  1 2.12E-06 7.66E-05 0.22 8.06
  2 2.53E-05 1.85E-04 2.66 19.45
  3 1.06E-05 1.39E-04 1.11 14.62
  4 0.00E + 00 4.33E-05 0.00 4.56
Average 9.51E-06   1.00 11.67*
SEM    0.60 3.33
Ser645/T646     
Experiment Normalized peak area # Ratio
  Control Insulin Control Insulin
  1 1.50E-04 3.00E-04 0.52 1.05
  2 2.93E-04 5.89E-04 1.02 2.05
  3 3.01E-04 7.03E-04 1.05 2.45
  4 4.04E-04 1.10E-03 1.41 3.82
Average 2.87E-04   1.00 2.34*
SEM    0.18 0.58
Ser711     
Experiment Normalized peak area # Ratio
  Basal Insulin Basal Insulin
  1 4.15E-05 4.29E-05 0.56 0.58
  2 5.58E-05 2.36E-05 0.76 0.32
  3 7.55E-05 8.23E-05 1.02 1.12
  4 1.22E-04 6.52E-05 1.66 0.88
Average 7.37E-05   1.00 0.73
SEM    0.24 0.17
Ser760     
Experiment Normalized peak area # Ratio
  Basal Insulin Basal Insulin
  1 3.43E-05 2.13E-05 1.00 0.62
  2 3.59E-05 2.85E-05 1.04 0.83
  3 2.84E-05 2.83E-05 0.83 0.82
  4 3.89E-05 2.08E-05 1.13 0.60
Average 3.44E-05   1.00 0.72
SEM    0.06 0.06
Ser839     
Experiment Normalized peak area # Ratio
  Basal Insulin Basal Insulin
  1 1.36E-05 1.67E-05 0.16 0.20
  2 2.74E-05 5.48E-05 0.33 0.66
  3 3.23E-05 0.00E + 00 0.39 0.00
  4 2.61E-04 2.70E-04 3.12 3.24
Average 8.36E-05   1.00 1.02
SEM    0.71 0.75
  1. # Normalized peak area: obtained by dividing the peak area of a phosphopeptide by the mean value of the peak area of the 8 unphosphorylated representative peptides of PPP1R12B.
  2. Ratio: obtained by dividing the normalized peak area for a phosphopeptide in each experiment by the average of the normalized peak area under the control condition.
  3. * P < 0.05 compared with control.