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Table 4 Effect of insulin on PPP1R12B phosphorylation, n = 4

From: Insulin-stimulated phosphorylation of protein phosphatase 1 regulatory subunit 12B revealed by HPLC-ESI-MS/MS

Ser29

    

Experiment

Normalized peak area #

Ratio †

 

Control

Insulin

Control

Insulin

  1

1.10E-05

3.89E-05

0.43

1.51

  2

1.20E-05

6.84E-05

0.46

2.65

  3

2.85E-05

5.64E-05

1.10

2.18

  4

5.18E-05

1.48E-04

2.01

5.75

Average

2.58E-05

 

1.00

3.02*

SEM

  

0.37

0.94

Ser504

    

Experiment

Normalized peak area #

Ratio †

 

Control

Insulin

Control

Insulin

  1

2.12E-06

7.66E-05

0.22

8.06

  2

2.53E-05

1.85E-04

2.66

19.45

  3

1.06E-05

1.39E-04

1.11

14.62

  4

0.00E + 00

4.33E-05

0.00

4.56

Average

9.51E-06

 

1.00

11.67*

SEM

  

0.60

3.33

Ser645/T646

    

Experiment

Normalized peak area #

Ratio †

 

Control

Insulin

Control

Insulin

  1

1.50E-04

3.00E-04

0.52

1.05

  2

2.93E-04

5.89E-04

1.02

2.05

  3

3.01E-04

7.03E-04

1.05

2.45

  4

4.04E-04

1.10E-03

1.41

3.82

Average

2.87E-04

 

1.00

2.34*

SEM

  

0.18

0.58

Ser711

    

Experiment

Normalized peak area #

Ratio †

 

Basal

Insulin

Basal

Insulin

  1

4.15E-05

4.29E-05

0.56

0.58

  2

5.58E-05

2.36E-05

0.76

0.32

  3

7.55E-05

8.23E-05

1.02

1.12

  4

1.22E-04

6.52E-05

1.66

0.88

Average

7.37E-05

 

1.00

0.73

SEM

  

0.24

0.17

Ser760

    

Experiment

Normalized peak area #

Ratio †

 

Basal

Insulin

Basal

Insulin

  1

3.43E-05

2.13E-05

1.00

0.62

  2

3.59E-05

2.85E-05

1.04

0.83

  3

2.84E-05

2.83E-05

0.83

0.82

  4

3.89E-05

2.08E-05

1.13

0.60

Average

3.44E-05

 

1.00

0.72

SEM

  

0.06

0.06

Ser839

    

Experiment

Normalized peak area #

Ratio †

 

Basal

Insulin

Basal

Insulin

  1

1.36E-05

1.67E-05

0.16

0.20

  2

2.74E-05

5.48E-05

0.33

0.66

  3

3.23E-05

0.00E + 00

0.39

0.00

  4

2.61E-04

2.70E-04

3.12

3.24

Average

8.36E-05

 

1.00

1.02

SEM

  

0.71

0.75

  1. # Normalized peak area: obtained by dividing the peak area of a phosphopeptide by the mean value of the peak area of the 8 unphosphorylated representative peptides of PPP1R12B.
  2. † Ratio: obtained by dividing the normalized peak area for a phosphopeptide in each experiment by the average of the normalized peak area under the control condition.
  3. * P < 0.05 compared with control.
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