Table 1 Protein identification by MALDI-TOF/TOF-MS of tryptic peptides obtained from 2-DE spots of T98G and U87MG glioma cell lines

1.34 ± 0.09

-

0.0053

UBIQ_HUMAN (8565/6.56)

Ubiquitin

302

61.8

1040.24

³⁴EGIPPDQQR⁴²

1068.35

⁶⁴ESTLHLVLR⁷²

1082.37

⁵⁵TLSDYNIQK⁶³

1525.84

³⁰IQDKEGIPPDQQR⁴²

1.92 ± 0.34

-

0.0422

S10AA_HUMAN (17259/9.59)

Protein S100-A10

58

10.3

1208.80

³⁸EFPGFLENQK⁴⁷

1.95 ± 0.52

-

0.0304

SSBP_HUMAN (17259/9.59)

Single-stranded DNA-binding protein, Mitochondrial

263

21.6

986.26

⁹⁶DVAYQYVK¹⁰³

1150.37

¹¹⁴IDYGEYMDK¹²²

1613.00

⁶⁷SGDSEVYQLGDVSQK⁸¹

1.87 ± 0.40

-

0.0356

PTMS_HUMAN (11530/4.14)

Parathymosin

67

10.8

1076.33

⁵SVEAAAELSAK¹⁵

1.37 ± 0.18

-

0.0474

TEBP_HUMAN (18982 / 4.35)

Prostaglandin E synthase 3

230

18.8

922.18

⁷²SILCCLR⁷⁸

1004.22

⁸⁰GESGQSWPR⁸⁸

1132.42

⁷⁹KGESGQSWPR⁸⁸

1446.70

³⁶LTFSCLGGSDNFK⁴⁸

1.39 ± 0.19

-

0.0454

TPIS_HUMAN (26954 / 6.45)

Triosephosphate isomerase

530

34.5

955.17

⁷FVGGNWK¹⁴

1138.43

⁶⁰IAVAAQNCYK⁶⁹

1235.51

¹⁹⁵SNVSDAVAQSTR²⁰⁶

1327.64

²⁰⁷IIYGGSVTGATCK2¹⁹

1467.77

¹⁷⁶TATPQQAQEVHEK¹⁸⁸

1587.88

⁸⁶DCGATWVVLGHSER⁹⁹

1639.98

⁷⁰VTNGAFTGEISPGMIK⁸⁵

2.40 ± 0.52

-

0.0351

TPM4_HUMAN (28636 / 4.67)

Tropomyosin alpha-4 chain

253

17.7

1015.23

⁴⁵AEGDVAALNR⁵⁴

1171.50

¹³³LVILEGELER¹⁴²

1244.53

⁵⁶IQLVEEELDR⁶⁵

1615.87

¹⁴IQALQQQADEAEDR²⁷

3.26 ± 0.47

-

0.0023

1433E_HUMAN (29345 / 4.63)

14-3-3 protein epsilon

519

28.2

908.21

⁴³NLLSVAYK⁵⁰

1206.57

²¹⁶DSTLIMQLLR²²⁵

1238.60

¹⁰⁷HLIPAANTGESK¹¹⁸

1257.58

¹³¹YLAEFATGNDR¹⁴¹

1385.78

¹³¹YLAEFATGNDRK¹⁴²

1448.74

³⁰VAGMDVELTVEER⁴²

1837.36

¹⁵⁴AASDIAMTELPPTHPIR¹⁷⁰

1.57 ± 0.32

-

0.0458

LDHB_HUMAN (36923 / 5.71)

L-lactate dehydrogenase B chain

372

16.8

914.25

⁹²IVVVTAGVR¹⁰⁰

960.27

³⁰⁰GLTSVINQK³⁰⁸

1177.46

³²⁰SADTLWDIQK³²⁹

1249.48

¹⁵⁹VIGSGCNLDSAR¹⁷⁰

1696.03

⁸LIAPVAEEEATVPNNK²³

2.19 ± 0.40

-

0.0330

ANXA1_HUMAN (38942 / 6.57)

Annexin A1

381

13.3

909.05

205ALYEAGER212

1214.35

167DITSDTSGDFR177

1263.42

114TPAQFDADELR124

1740.87

189SEDFGVNEDLADSDAR204

3.01 ± 1.08

-

0.0189

RINI_HUMAN(51799 / 4.71)

Ribonuclease inhibitor

579

18.7

1150.41

³⁵LDDCGLTEAR⁴⁴

1161.46

¹⁹⁶DSPCQLEALK²⁰⁵

1210.51

⁵⁴VNPALAELNLR⁶⁴

1347.66

³⁴²FLLELQISNNR³⁵²

1514.91

³⁶⁰ELCQGLGQPGSVLR³⁷³

1531.76

²⁸⁸ELSLAGNELGDEGAR³⁰²

1631.88

¹⁷⁴ELTVSNNDINEAGVR¹⁸⁸

1.51 ± 0.17

-

0.0388

STMN1_HUMAN (17302 / 5.76)

Stathmin

219

29.5

1075.39

⁴⁴DLSLEEIQK⁵²

1166.46

⁸⁶AIEENNNFSK⁹⁵

1327.75

³⁰ESVPEFPLSPPK⁴¹

1389.81

¹⁵ASGQAFELILSPR²⁷

2.99 ± 1.16

-

0.0194

PRDX6_HUMAN (25149 / 6.0)

Peroxiredoxin-6

354

22.8

907.12

¹⁵⁶NFDEILR¹⁶²

916.19

¹⁷⁵VATPVDWK¹⁸²

1192.53

¹⁴⁵LSILYPATTGR¹⁵⁵

1396.66

⁴²DFTPVCTTELGR⁵³

1583.75

⁸⁵DINAYNCEEPTEK⁹⁷

1.39 ± 0.14

-

0.0311

PDIA3_HUMAN (57181 / 5.98)

Protein disulfide-isomerase A3

449

15.8

996.28

¹³¹QAGPASVPLR¹⁴⁰

1085.33

⁹⁵YGVSGYPTLK¹⁰⁴

1173.40

³³⁶FVMQEEFSR³⁴⁴

1192.44

⁶³LAPEYEAAATR⁷³

1237.40

¹⁰⁸DGEEAGAYDGPR¹¹⁹

1369.62

³⁶⁷SEPIPESNDGPVK³⁷⁹

1681.91

⁴³⁴MDATANDVPSPYEVR⁴⁴⁸

-

2.33 ± 0.51

0.0099

MIF_HUMAN(12647 / 7.74)

Macrophage migration inhibitory factor

79

17.4

1045.37

79LLCGLLAER87

1304.69

2PMFIVNTNVPR12

-

1.83 ± 0.28

0.0070

PPIA_HUMAN (18240 / 7.68)

Peptidyl-prolyl cis-trans isomerase A

337

32.7

1056.15

20VSFELFADK28

1155.24

⁸³FEDENFILK⁹¹

1248.37

¹⁵⁵KITIADCGQLE¹⁶⁵

1295.21

¹³⁴EGMNIVEAMER¹⁴⁴

1615.52

⁵⁶IIPGFMCQGGDFTR⁶⁹

-

3.62 ± 0.65

0.0022

PRDX1_HUMAN (22338 / 8.27)

Peroxiredoxin-1

178

19.6

921.18

¹²⁹GLFIIDDK¹³⁶

1108.45

¹¹¹TIAQDYGVLK¹²⁰

1197.56

¹⁵⁹LVQAFQFTDK¹⁶⁸

1212.59

¹⁴¹QITVNDLPVGR¹⁵¹

-

2.30 ± 0.31

0.0009

EF1D_HUMAN (31236 / 4.9)

Elongation factor 1-delta

458

24.6

974.13

²³³LVPVGYGIR²⁴¹

1088.16

³⁹QENGASVILR⁴⁸

1359.47

⁸⁴IASLEVENQSLR⁹⁵

1528.59

²⁵FYEQMNGPVAGASR³⁸

-

2.10 ± 0.60

0.0214

HNRPC_HUMAN (33727 / 4.95)

Heterogeneous nuclearribonucleoproteins C1/C2

123

8.8

904.11

¹³⁶MYSYPAR¹⁴²

944.28

¹⁴³VPPPPPIAR¹⁵¹

1330.71

⁵¹GFAFVQYVNER⁶¹

-

1.87 ± 0.47

0.0476

SET_HUMAN(33488 / 4.23)

Protein SET

290

11.7

1209.45

¹²³VEVTEFEDIK¹³²

1274.57

⁵⁸LNEQASEEILK⁶⁸

1447.63

¹⁵⁵EFHLNESGDPSSK¹⁶⁷

-

3.87 ± 1.03

0.0340

G6PD_HUMAN (59712 / 6.39)

Glucose-6-phosphate1-dehydrogenase

516

15.7

947.20

¹⁰TQVCGILR¹⁷

1003.28

²²⁰IFGPIWNR²²⁷

1059.38

¹⁶⁷IIVEKPFGR¹⁷⁵

1133.39

³²¹GYLDDPTVPR³³⁰

1174.51

¹⁸³LSNHISSLFR¹⁹²

1192.42

⁴⁹⁹VGFQYEGTYK⁵⁰⁸

1233.58

²⁰⁶EMVQNLMVLR²¹⁵

1809.05

¹⁰⁵NSYVAGQYDDAASYQR¹²⁰

-

3.20 ± 1.20

0.0491

TBA1A_HUMAN (50820 / 4.94)

Tubulin alpha-1A chain

390

14.9

904.06

³⁹⁵FDLMYAK⁴⁰¹

1016.30

³²⁷DVNAAIATIK³³⁶

1086.36

¹¹³EIIDLVLDR¹²¹

1250.36

³¹²YMACCLLYR³²⁰

1719.98

²¹⁶NLDIERPTYTNLNR229

1826.21

³⁵³VGINYQPPTVVPGGDLAK³¹⁰

-

3.73 ± 1.52

0.0338

GRP78_HUMAN (72446 / 5.07)

78 kDa glucose-regulated protein

830

20.5

987.10

533LTPEEIER540

1192.36

⁴⁶⁵VYEGERPLTK⁴⁷⁴

1212.32

³⁷⁷EFFNGKEPSR3⁸⁶

1218.36

¹⁸⁶DAGTIAGLNVMR¹⁹⁷

1229.35

⁵⁰VEIIANDQGNR⁶⁰

1317.44

⁵⁶³NELESYAYSLK573

1431.53

¹⁰²TWNDPSVQQDIK¹¹³

1461.64

³⁵⁴SDIDEIVLVGGSTR³⁶⁷

1567.70

⁶¹ITPSYVAFTPEGER⁷⁴

1678.79

⁸²NQLTSNPENTVFDAK⁹⁶

1838.01

⁴⁴⁸SQIFSTASDNQPTVTIK⁴⁶⁴

-

1.69 ± 0.29

0.0224

GRP75_HUMAN (73965 / 5.87)

Stress-70 protein, mitochondrial

545

11.5

1243.42

²⁰⁷DAGQISGLNVLR²¹⁸

1291.49

³⁹⁵VQQTVQDLFGR⁴⁰⁵

1362.58

³⁴⁹AQFEGIVTDLIR³⁶⁰

1451.59

⁸⁶TTPSVVAFTADGER⁹⁹

1463.67

³⁷⁸SDIGEVILVGGMTR³⁹¹

1695.86

¹⁸⁸NAVITVPAYFNDSQR²⁰²