Figure 1From: Technical updates to basic proteins focalization using IPG strips 2DE separation of MCF7 basic protein: iso-electric focalisation was performed with 50 μg proteins using 6–11 pH IPG strip and 12% acrylamide was used for the second dimension. Proteins were loaded by in-gel rehydration (A), anodic cup loading (B), and paper-bridge loading (C).Back to article page