Table 1 Methods tested for basic proteins focalization with the corresponding number of spots in area A and B (Figure 3 ) and an estimation of each focalization efficiency and overall image quality
From: Technical updates to basic proteins focalization using IPG strips
Method | Strip rehydration | Cathodic wicks | Spots area A | Spots area B | Focalisation efficiency | Overall image quality |
|---|---|---|---|---|---|---|
1 | 160 mM DTT | 50 μl water | 82 +/− 9,9 | 79 +/− 14,1 | +/− | -- |
2 | 160 mM DTT | 50 μl RB | 73 +/− 7,1 | 76,5 +/− 3,5 | - | - |
3 | 160 mM DTT | 50 μl RB | 67 +/− 4,2 | 78 +/− 4,2 | +/− | +/− |
160 mM DTT | ||||||
4 | 160 mM DTT | 50 μl water | 82 +/−8,5 | 76,5 +/− 4,9 | +/− | -- |
10% isopropanol | ||||||
5 | 160 mM DTT | 50 μl RB | 60 +/− 11,3 | 70,5 +/− 9,2 | - | - |
10% isopropanol | ||||||
6 | 160 mM DTT | 50 μl RB | 67,5 +/− 9,2 | 80,5 +/− 0,7 | +/− | +/− |
10% isopropanol | 160 mM DTT | |||||
7 | 1,2% destreak reagent | 50 μl water | 58 +/− 1,4 | 61,5 +/− 4,9 | +/− | +/− |
8 | 1,2% destreak reagent | 50 μl RB | 40 +/− 24 | 67 +/− 17 | + | - |
9 | 1,2% destreak reagent | 50 μl RB | 71,5 +/− 0,7 | 72,5 +/− 3,5 | ++ | ++ |
160 mM DTT | ||||||
10 | 1,2% destreak reagent | 50 μl water | 35 +/− 1,4 | 65,5 +/− 4,9 | +/− | +/− |
10% isopropanol | ||||||
11 | 1,2% destreak reagent | 50 μl RB | 36,5 +/− 3,5 | 59 +/− 5,7 | + | - |
10% isopropanol | ||||||
12 | 1,2% destreak reagent | 50 μl RB | 72 +/− 1,4 | 74 +/− 7,1 | ++ | ++ |
10% isopropanol | 160 mM DTT |