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Table 1 Methods tested for basic proteins focalization with the corresponding number of spots in area A and B (Figure 3 ) and an estimation of each focalization efficiency and overall image quality

From: Technical updates to basic proteins focalization using IPG strips

Method Strip rehydration Cathodic wicks Spots area A Spots area B Focalisation efficiency Overall image quality
1 160 mM DTT 50 μl water 82 +/− 9,9 79 +/− 14,1 +/− --
2 160 mM DTT 50 μl RB 73 +/− 7,1 76,5 +/− 3,5 - -
3 160 mM DTT 50 μl RB 67 +/− 4,2 78 +/− 4,2 +/− +/−
   160 mM DTT     
4 160 mM DTT 50 μl water 82 +/−8,5 76,5 +/− 4,9 +/− --
  10% isopropanol      
5 160 mM DTT 50 μl RB 60 +/− 11,3 70,5 +/− 9,2 - -
  10% isopropanol      
6 160 mM DTT 50 μl RB 67,5 +/− 9,2 80,5 +/− 0,7 +/− +/−
  10% isopropanol 160 mM DTT     
7 1,2% destreak reagent 50 μl water 58 +/− 1,4 61,5 +/− 4,9 +/− +/−
8 1,2% destreak reagent 50 μl RB 40 +/− 24 67 +/− 17 + -
9 1,2% destreak reagent 50 μl RB 71,5 +/− 0,7 72,5 +/− 3,5 ++ ++
   160 mM DTT     
10 1,2% destreak reagent 50 μl water 35 +/− 1,4 65,5 +/− 4,9 +/− +/−
  10% isopropanol      
11 1,2% destreak reagent 50 μl RB 36,5 +/− 3,5 59 +/− 5,7 + -
  10% isopropanol      
12 1,2% destreak reagent 50 μl RB 72 +/− 1,4 74 +/− 7,1 ++ ++
  10% isopropanol 160 mM DTT