Differential protein profile of sequentially depleted serum. One-dimensional (A) and two-dimensional (B) gel electrophoresis separations, showing the different protein profiles of non-depleted human serum (ND) and serum depleted (D) according to the sequential method presented in this work. Some of the most representative proteins that are either depleted or typically present in the depleted maps are depicted. A1AT: alpha-1-antitrypsin; A2MG: alpha-2-macroglobulin; ALBU: serum albumin; APOA1: apolipoprotein A1; APOC3: apolipoprotein C3; CO3α: complement factor α; HPT: haptoglobin; IGGγ: IgG heavy (gamma) chain; IGGκ/λ: IgG light (kappa/lambda) chains; TRFE: transferrin; TTYH: transthyretin. In (C), this protein profile is compared to those obtained with ACN and DTT protein depletion methods alone. 1: crude serum, 2: ACN depletion, 3: DTT depletion, 4: sequential depletion.