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Table 3 Proteins identified in this work as altered in Osteoarthritis vs Control sera

From: Sequential depletion of human serum for the search of osteoarthritis biomarkers

Protein ID a) Acc.No. a) Name Function Ratio OA:N b) Anova (p) Predicted c) Experimental d) Score e) Sequence tags f)
MW (Da) pI MW (Da) pI
A1AT P01009 Alpha-1-antitrypsin Inhibitor of serine proteases 1.4 0.008 46878 5.37 40047 5.33 151 K. TDTSHHDQDHPTFNK.I
            K. ITPNLAEFAFSLYR.Q
            K. QINDYVEKGTQGK.I
            K. GKWERPFEVK.D
            K. DTEEEDFHVDQVTTVK.V
            R. LGMFNIQHCK.K
            K. LQHLENELTHDIITK.F
            K. FLENEDRR.S
            K. VFSNGADLSGVTEEAPLK.L
            K. FNKPFVFLMIEQNTK.S
FETUA P02765 Alpha-2-HS-glycoprotein Negative regulation of bone mineralization 2 0.004 40098 5.43 40098 5.43 102 K. CNLLAEKQYGFCK.A
        R. HTFMGVVSLGSPSGEVSHPR.K
ANGT P01019 Angiotensinogen Essential component of the renin-angiotensin system (RAS) 1.3 0.001 53406 5.87 40056 5.48 28 K. ANAGKPKDPTFIPAPIQAK.T
APOA1 P02647 Apolipoprotein A-I Cholesterol metabolism 2.2 0.013 30759 5.56 31221 5.43 271 K. DSGRDYVSQFEGSALGK.Q
            K. LLDNWDSVTSTFSK.L
            K. LLDNWDSVTSTFSKLR.E
            K. VQPYLDDFQKK.W
            K. KWQEEMELYR.Q
            K. WQEEMELYR.Q
            K. VEPLRAELQEGAR.Q
            K. LSPLGEEMRDR.A
            R. THLAPYSDELR.Q
            R. THLAPYSDELRQR.L
            K. ATEHLSTLSEKAKPALEDLR.Q
            K. AKPALEDLR.Q
APOA2 P02652 Apolipoprotein A-II Lipid transport 0.7 0.003 11282 6.26 11064 5.23 82 K. VKSPELQAEAK.S
            K. SPELQAEAK.S
            K. SKEQLTPLIK.K
APOA4 P06727 Apolipoprotein A-IV Lipid transport 0.6 0.005 45371 5.28 37738 5.47 37 K. LVPFATELHER.L
            R. LEPYADQLR.T
            R. TQVNTQAEQLRR.Q
            R. QLTPYAQR.M
            K. IDQNVEELKGR.L
            R. ISASAEELRQR.L
            R. LAPLAEDVR.G
            R. LAPLAEDVRGNLR.G
            K. SLAELGGHLDQQVEEFR.R
            K. SLAELGGHLDQQVEEFRR.R
            R. RVEPYGENFNK.A
            K. ALVQQMEQLR.Q
APOC3 P02656 Apolipoprotein C-III Lipid degradation and transport 0.8 0.014 10846 5.23 13922 5.16 149 K. TAKDALSSVQESQVAQQAR.G
            K. DALSSVQESQVAQQAR.G
HPT P00738 Haptoglobin (β chain) Cellular iron ion homeostasis 2 0.007 45861 6.13 35377 5.74 90 K. GSFPWQAK.M
        R. NANFKFTDHLK.Y
            K. YVMLPVADQDQCIR.H
            K. SPVGVQPILNEHTFCAGMSK.Y
HPT P00738 Haptoglobin (α 1 chain) Cellular iron ion homeostasis 1.4 0.006 45861 6.13 19281 5.98 68 R. YQCKNYYK.L
        K. LRTEGDGVYTLNNEK.Q
            K. AVGDKLPECEAVCGKPK.N
HPT P00738 Haptoglobin (α 2 chain) Cellular iron ion homeostasis 0.5 0.0002 45861 6.13 28110 5.97 135 R. TEGDGVYTLNDKK.Q
        K. LRTEGDGVYTLNNEK.Q
            K. AVGDKLPECEAVCGKPK.N
            K. AVGDKLPECEAVCGKPK.N
HBB P68871 Hemoglobin subunit beta Involved in oxygen transport from the lung to peripheral tissues 1.6 0.014 16102 6.75 16102 6.75 43 R. LLVVYPWTQR.F
IGKC P01834 Ig kappa chain C region Immune response 0.6 0.003 11773 5.58 33273 6.42 107 -. TVAAPSVFIFPPSDEQLK.S
            K. SGTASVVCLLNNFYPR.E
LAC2 P0CG05 Ig lambda-2 chain C regions Complement activation, classical pathway 0.6 0.003 11458 6.92 33273 6.42 72 K. AAPSVTLFPPSSEELQANK.A
        K. QSNNKYAASSYLSLTPEQWK.S
            R. SYSCQVTHEGSTVEK.T
            R. SYSCQVTHEGSTVEKTVAPTECS.-
A2GL P02750 Leucine-rich alpha-2-glycoprotein Protein binding (secreted) 1.5 0.005 38382 6.45 38507 5.47 88 R. YLFLNGNKLAR.V
        R. VAAGAFQGLR.Q
            R. CAGPEAVKGQTLLAVAK.S
SAA P02735 Serum amyloid A protein Acute-phase response 0.8 0.014 13581 6.28 15299 6.53 52 R.EANYIGSDKYFHAR.G
            R.FFGHGAEDSLADQAANEWGR.S
TTHY P02766 Transthyretin Thyroid hormone-binding protein 2.2 0.022 15991 5.52 28287 6.3 136 R.KAADDTWEPFASGK.T
            K.AADDTWEPFASGK.T
  1. a) Protein ID and accession number according to SwissProt database.
  2. b) Average volume ratio OA:N, quantified by SameSpots software.
  3. c) Predicted molecular weight (MW) and Isoelectric point (pI) according to protein sequence and SwissProt database.
  4. d) Experimental molecular weight (MW) and Isoelectric point (pI) calculated by analysis of the gel images with SameSpots software.
  5. e) MS protein score obtained from MALDI-TOF/TOF spectra using Mascot search engine.
  6. f) Sequence tags identified by MALDI-TOF/TOF MS/MS.