Figure 3From: An efficient procedure for protein extraction from formalin-fixed, paraffin-embedded tissues for reverse phase protein arrays Phosphorylation patterns of the AKT pathway according to cell treatment and preparation type. Phospho-AKT and phospho-S6 were detected using RPPA. The lysates spotted on the protein arrays were derived from breast and ovarian cancer cell lines treated with growth factors or inhibitors. Starved = serum-starved cell cultures; EGF = growth factor treated; LY = LY294002; PD = PD98059.Back to article page