Table 1 Protein extraction methods
Method 1 | Method 2 | Method 3 | Method 4 | Method 5 | Method 6 | Qiagen | |
|---|---|---|---|---|---|---|---|
RPPA buffer | ✓ | ✓ | ✓ | ||||
Qproteome buffer | ✓ | ||||||
20mM Tris–HCl, pH=9 | ✓ | ||||||
20mM Tris–HCl, pH=7.4 | ✓ | ||||||
20mM Tris–HCl, pH=4 | ✓ | ||||||
sonication | ✓ | ✓ | |||||
incubation at 80 o C | ✓ | ✓ | ✓ | ✓ | ✓ |