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Table 1 Protein extraction methods

From: An efficient procedure for protein extraction from formalin-fixed, paraffin-embedded tissues for reverse phase protein arrays

  Method 1 Method 2 Method 3 Method 4 Method 5 Method 6 Qiagen
RPPA buffer     
Qproteome buffer       
20mM Tris–HCl, pH=9        
20mM Tris–HCl, pH=7.4        
20mM Tris–HCl, pH=4        
sonication       
incubation at 80 o C    
  1. The differences in buffer type and conditions between the protocols for protein extraction from FFPE cells and tissues used in the study are shown.