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Table 2 Distribution of RPPA-detected protein markers depending on their performance between frozen and FFPE preparations

From: An efficient procedure for protein extraction from formalin-fixed, paraffin-embedded tissues for reverse phase protein arrays

  Markers with p < 0.05 between FFPE and FF Markers with large intensity difference between FFPE and FF Markers with low intensity in both FFPE and FF Markers with low replicate reproducibility (R < 0.7)
Cell lines 77 (45%) 54 (32%) 28 (17%) 10 (6%)
Breast cancer set 54 (32%) 81 (48%) 25 (15%) 9 (5%)
Renal cancer set 107 (64%) 31 (18%) 7 (4%) 24 (14%)
  1. Markers with high correlation (p < 0.05) between frozen and FFPE preparations, large intensity differences, low signal or low reproducibility for cell lines, breast cancer set and renal set are shown.