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Table 2 Distribution of RPPA-detected protein markers depending on their performance between frozen and FFPE preparations

From: An efficient procedure for protein extraction from formalin-fixed, paraffin-embedded tissues for reverse phase protein arrays

 

Markers with p < 0.05 between FFPE and FF

Markers with large intensity difference between FFPE and FF

Markers with low intensity in both FFPE and FF

Markers with low replicate reproducibility (R < 0.7)

Cell lines

77 (45%)

54 (32%)

28 (17%)

10 (6%)

Breast cancer set

54 (32%)

81 (48%)

25 (15%)

9 (5%)

Renal cancer set

107 (64%)

31 (18%)

7 (4%)

24 (14%)

  1. Markers with high correlation (p < 0.05) between frozen and FFPE preparations, large intensity differences, low signal or low reproducibility for cell lines, breast cancer set and renal set are shown.
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Proteome Science

ISSN: 1477-5956