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Table 1 Proteins identified in membrane fraction using 2-DE coupled with MS/MS analysis

From: Proteomic analysis reveals the diversity and complexity of membrane proteins in chickpea (Cicer arietinum L.)

Functional Category Spot No. a Identification Score gi No. b NP c GRAVY Value d % Coverage Theoretical MW/pI Experimental MW/pI
Signaling and stress response CaM-89* Serine threonine kinase homolog COK-4 48 9796478 2 −0.036 5 42.25/6.42 44.78/4.74
  CaM-246! Putative serine/threonine kinase 71 110289142 11 −0.526 25 50.40/9.89 25.76/5.30
  CaM-247¶ Membrane protein CH1-like 41 115441379 3 −0.510 2 70.30/4.91 30.04/5.45
  CaM-85! Calcineurin-like phospho-esterase family protein 63 22329383 11 −0.370 29 43.99/5.36 42.64/4.72
  CaM-436* Putative quinone oxidoreductase 158 21068664 4 −0.124 25 21.70/6.51 28.26/6.62
  CaM-73* Actin (Fragment) 63 111610552 1 −0.165 7 23.51/4.78 26.43/4.75
  CaM-428* Beta-1,3-glucanase 80 116490100 1 0.060 3 36.41/8.82 24.77/6.75
  CaM-182* Ferritine 50 224140479 1 −0.284 6 16.58/5.14 28.10/5.12
  CaM-487* Guanine nucleotide-binding protein subunit beta-like 297 3023847 7 −0.153 30 31.83/6.44 36.41/6.89
  CaM-191* Ferritine 129 255637227 2 −0.297 9 30.24/5.23 30.24/5.23
  CaM-326¶ Cytochrome P450 32 15238866 2 −0.218 4 58.91/7.98 52.62/5.68
  CaM-432¶ Disease resistance protein (Fragment) 32 108739945 2 −0.119 4 23.29/6.10 21.25/6.62
  CaM-94* Chaperonin 60 alphaf subunit 764 217074850 17 0.004 28 62.02/5.11 72.03/4.7
  CaM-99* Chaperone DnaK 501 92870233 11 −0.330 15 75.71/5.19 83.09/4.69
  CaM-483* Luminal binding protein, BiP 248 297742397 7 −0.374 6 73.31/4.96 30.25/6.91
  CaM-157* Putative GloEL protein Chaperonin 60 kDa 192 225435794 4 −0.094 38 17.51/10.05 45.83/5.00
  CaM-150! Chaperonin 60 alphaf subunit 108 3790441 12 0.001 13 61.45/5.23 57.09/5.12
  CaM-421¶ Chaperonin 60 alphaf chain precursor, 78 1710807 4 −0.025 7 61.99/5.15 85.92/6.28
Protein synthesis and degradation CaM-39* Putative 60S ribosomal protein L1 101 84468414 3 −0.445 7 44.93/10.40 51.00/4.29
  CaM-240* 40S ribosomal protein S12 54 255626071 2 0.106 12 15.34/5.50 19.26/5.37
  CaM-283* 40S ribosomal protein S12 162 255626071 3 0.106 23 15.345/5.50 19.02/5.607
  CaM-223! Putative glutamate-tRNA ligase 70 115443869 14 −0.304 11 63.10/6.27 74.10/5.23
  CaM-208* Putative 60S acidic ribosomal protein P0 89 84468360 2 0.031 7 34.31/5.27 40.24/5.10
  CaM-209* Putative 60S acidic ribosomal protein P0 82 84468360 2 0.031 7 34.31/5.27 40.25/5.21
  CaM-301* Proteasome subunit alpha type 199 224139394 4 −0.227 21 27.38/5.59 31.39/5.81
  CaM-249! Polyubiquitin 64 18421671 10 −0.281 27 36.40/5.83 28.56/5.47
  CaM-407! Mitochondrial processing peptidase beta subunit 394 12802327 20 −0.302 22 58.90/6.56 70.46/6.03
Bioenergy CaM-242* Cytochrome b6-f complex iron-sulfur subunit 76 136707 2 −0.039 15 16.85/6.45 22.19/5.53
  CaM-21! Chlorophyll a/b-bindingg protein AB96 224 115773 9 0.011 22 24.36/4.98 29.51/4.58
  CaM-75* Chlorophyll a/b bindingg protein 411 3928140 12 −0.068 30 28.35/5.47 30.58/4.78
  CaM-196* Oxygen-evolving enhancer protein 1h 177 131384 6 −0.303 15 35.44/5.19 35.44/5.19
  CaM-294* Putative PSII-P protein 104 217072770 2 −0.273 8 28.41/7.12 25.41/5.59
  CaM-22! Chlorophyll a/b-binding protein 3 precursorg 160 115800 7 0.005 19 27.90/5.46 29.38/4.47
  CaM-69! Chlorophyll A-B binding proteing 118 169124051 8 0.071 12 28.59/5.44 28.05/4.62
  CaM-126! Chlorophyll a/b-binding protein type III (Fragment)g 65 7271947 4 −0.013 9 20.81/5.17 26.40/4.95
  CaM-127! PSI type III chlorophyll a/b-binding protein (imported) 99 15219941 7 −0.014 11 29.16/8.61 27.98/4.98
  CaM-125! Oxygen-evolving enhancer protein 1h 111 131384 7 −0.303 14 35.10/6.25 27.83/4.87
  CaM-183! Oxygen-evolving enhancer protein 2 89 131390 9 −0.312 14 28.20/8.29 25.64/5.16
  CaM-376* Ferredoxin NADP reductase 81 125553745 2 −0.340 5 39.95/8.72 36.9/6.12
  CaM-184¶ LHCA3, chlorophyll binding 58 79320443 4 −0.009 18 23.84/5.62 28.07/5.23
  CaM-74! Light-harvesting chlorophyll-a/b binding protein Lhcb1 387 56809379 13 −0.083 19 28.43/5.48 30.58/4.93
  CaM-319! Putative ferredoxin-NADP(H) oxidoreductase 67 41052915 7 −0.383 19 41.09/7.98 39.76/5.92
  CaM-452¶ Ferredoxin: NADP + reductase 130 4930119 3 −0.403 12 34.98/6.54 36.58/6.49
  CaM-226! ATPase beta subunit (Fragment)i 276 6467935 18 −0.026 27 51.53/5.22 65.89/5.21
  CaM-147* ATP synthase subunit beta, chloroplastici 92 11466372 2 0.007 6 51.99/5.10 44.87/4.84
  CaM-189* ATP synthase subunit alphaj 589 197294119 18 −0.057 24 55.64/5.44 32.38/5.16
  CaM-221! ATP synthase beta subunit (Fragment)i 116 3850926 13 0.001 22 52.73/5.15 65.80/5.14
  CaM-161! ATP synthase beta subunit (Fragment)i 918 7708546 41 0.004 51 51.18/5.07 65.76/5.04
  CaM-80! ATP synthase beta subunit (Fragment)i 671 4063542 19 0.009 26 49.13/5.06 35.75/4.60
  CaM-316* ATP synthase subunit alphaj 328 197294119 10 −0.057 17 55.64/5.44 45.62/5.83
  CaM-222* ATP synthase subunit betai 410 197294097 19 −0.016 27 52.96/5.16 73.99/5.16
  CaM-335* ATP synthase subunit alphaj 75 197294119 2 −0.057 4 55.64/5.44 69.22/5.62
  CaM-385¶ ATP synthase subunit gamma, chloroplast precursor 77 226533016 1 −0.143 3 40.10/8.44 41.00/6.06
  CaM-311* ATP synthase CF1 alpha subunitj 295 289066833 5 −0.045 11 55.73/5.21 42.49/5.89
  CaM-396* ATP synthase subunit alphaj 439 197294119 10 −0.057 19 55.64/5.44 52.90/5.91
  CaM-272! ATP synthase CF1 alpha subunitj 572 13518443 21 −0.055 23 55.80/5.22 69.51/5.51
  CaM-271! H + −transporting two-sector ATPase alpha chain 83 114522 13 −0.044 21 54.64/5.75 69.09/5.38
Metabolism CaM-218* Glutamine synthetase 50 12963877 1 −0.393 2 47.40/5.73 50.72/5.32
  CaM-308* Fructose-bisphosphate aldolase 1k 182 399024 5 −0.172 14 38.63/5.83 39.99/5.62
  CaM-310¶ Fructose-bisphosphate aldolase (EC 4.1. 2.13) precursork 206 399024 6 −0.172 17 38.74/5.83 39.89/5.86
  CaM-453* Fructose-bisphosphate aldolasek 122 3913008 4 −0.148 14 38.42/6.21 37.10/6.70
  CaM-322* Phosphoglycerate kinase precursor like 124 82621134 5 0.128 11 50.35/8.19 46.94/5.64
  CaM-257¶ Plastidic aldolasek 240 38096041 9 −0.151 17 43.18/6.86 35.52/5.61
  CaM-210* Fructose-bisphosphate aldolasek 163 84468290 5 −0.140 14 43.03/6.86 40.35/5.35
  CaM-460! Glyceraldehyde 3-phosphate dehydrogenasel 70 3413165 4 0.069 15 20.88/5.24 41.91/6.66
  CaM-216¶ Glutamate-ammonia ligase (EC 6.3.1.2) delta precursor 39 121344 1 −0.390 2 47.65/6.18 51.86/5.13
  CaM-115! Trypsin inhibitor, chain B (fragments) 144 3318877 7 −0.281 57 19.29/4.79 14.86/5.07
  CaM-214¶ Glutamate-ammonia ligase (EC 6.3.1.2) delta precursor 68 121344 1 −0.390 2 47.65/6.18 51.14/5.23
  CaM-366* Carbonic anhydrasem 55 20502881 2 −0.159 4 35.28/6.96 31.01/5.95
  CaM-370* Carbonic anhydrasem 67 20502881 2 −0.159 5 35.28/6.96 30.85/6.23
  CaM-206! Phosphoribulokinase 71 1885326 6 −0.289 7 39.23/5.41 43.82/5.18
  CaM-435* Carbonic anhydrasem 95 8954289 3 −0.091 6 35.46/7.59 29.73/6.47
  CaM-438* Carbonic anhydrasem 56 8954289 2 −0.091 4 35.46/7.59 29.78/6.74
  CaM-462* Glyceraldehyde-3-phosphate dehydrogenase Al 200 120658 6 −0.047 12 43.31/8.80 44.49/6.81
Miscellaneous CaM-155! Dynein-1-alpha heavy chain 69 159490411 63 −0.257 10 525.42/5.32 61.98/5.11
  CaM-54! Activator of spomin 65 145357734 8 −0.634 24 17.25/9.63 12.38/4.69
  CaM-289¶ Ribulose-1,5-biphosphate carboxylase/oxygenase large subunit 129 10764577 5 −0.254 10 51.58/5.96 23.69/5.59
  CaM-299! Histone acetyltransferase GCN5 79 18410098 15 −0.581 26 63.48/6.01 30.69/5.63
  CaM-38* Ribulose bisphosphate carboxylase/Oxygenase large chain 60 1750362 2 −0.249 4 52.22/6.09 56.17/4.30
  CaM-138* Chromosome chr17 scaffold_16 149 225456471 4 −0.342 4 33.35/5.67 37.90/4.97
  CaM-43* Ribulose bisphosphate carboxylase large chain 295 197294096 8 −0.277 16 52.65/6.04 64.35/4.29
  CaM-290! Intronic ORF, similar to LAGLIDADG endonuclease 80 2943730 11 −0.486 21 29.46/10.01 21.47/6.00
Unknown function CaM-443* Predicted protein 53 224119706 1 0.038 3 27.44/8.65 31.20/6.64
  CaM-263¶ OSJNBb0085H11.1 protein 50 38346013 1 -.293 7 176.76/9.26 53.68/5.61
  CaM-478* Putative uncharacterized protein 64 147828109 19 −0.788 0 58.79/6.46 87.91/6.46
  CaM-323* Putative uncharacterized protein 94 224284512 2 −0.395 7 40.97/6.04 44.21/5.66
  CaM-477! Unknown protein 67 27497205 16 −1.250 8 16.57/10.81 65.11/6.62
  CaM-171! Unknown protein 67 15236812 16 −0.827 8 90.90/5.09 74.93/5.11
  CaM-64! Hypothetical protein P0415D04.53 66 47496995 8 −0.587 34 30.31/11.81 24.12/4.77
  CaM-267! Hypothetical protein F26P21.180 67 3688187 10 −0.645 22 57.25/7.64 44.54/5.58
  CaM-56! Hypothetical protein OSJNBa0075N02.148 66 28972006 7 −1.039 29 20.62/12.24 22.37/4.49
  1. aSpot number as marked on the proteome (Figure 3B). The spot numbers are designated as CaM-X, where Ca indicates the organism ( C icer a rietinum), M denotes the fraction (m embrane), and X corresponds to the spot number. bProtein identification number as in GenBank. cNP represents the number of peptides. dGRAVY value calculated using ProtParam tool available on ExPasy server. e-m denotes the respective protein(s) identified from multiple spots. This could be caused by posttranslational modification(s) or different isoforms, protein degradation/synthesis. Spots marked by ‘*’ were identified by Q-TRAP, ‘¶’ by QStar, and ‘!’ by MALDI TOF-TOF analysis.