Table 1 Proteins identified in membrane fraction using 2-DE coupled with MS/MS analysis

Signaling and stress response

CaM-89*

Serine threonine kinase homolog COK-4

48

9796478

2

−0.036

5

42.25/6.42

44.78/4.74

CaM-246!

Putative serine/threonine kinase

71

110289142

11

−0.526

25

50.40/9.89

25.76/5.30

CaM-247¶

Membrane protein CH1-like

41

115441379

3

−0.510

2

70.30/4.91

30.04/5.45

CaM-85!

Calcineurin-like phospho-esterase family protein

63

22329383

11

−0.370

29

43.99/5.36

42.64/4.72

CaM-436*

Putative quinone oxidoreductase

158

21068664

4

−0.124

25

21.70/6.51

28.26/6.62

CaM-73*

Actin (Fragment)

63

111610552

1

−0.165

7

23.51/4.78

26.43/4.75

CaM-428*

Beta-1,3-glucanase

80

116490100

1

0.060

3

36.41/8.82

24.77/6.75

CaM-182*

Ferritin^e

50

224140479

1

−0.284

6

16.58/5.14

28.10/5.12

CaM-487*

Guanine nucleotide-binding protein subunit beta-like

297

3023847

7

−0.153

30

31.83/6.44

36.41/6.89

CaM-191*

Ferritin^e

129

255637227

2

−0.297

9

30.24/5.23

30.24/5.23

CaM-326¶

Cytochrome P450

32

15238866

2

−0.218

4

58.91/7.98

52.62/5.68

CaM-432¶

Disease resistance protein (Fragment)

32

108739945

2

−0.119

4

23.29/6.10

21.25/6.62

CaM-94*

Chaperonin 60 alpha^f subunit

764

217074850

17

0.004

28

62.02/5.11

72.03/4.7

CaM-99*

Chaperone DnaK

501

92870233

11

−0.330

15

75.71/5.19

83.09/4.69

CaM-483*

Luminal binding protein, BiP

248

297742397

7

−0.374

6

73.31/4.96

30.25/6.91

CaM-157*

Putative GloEL protein Chaperonin 60 kDa

192

225435794

4

−0.094

38

17.51/10.05

45.83/5.00

CaM-150!

Chaperonin 60 alpha^f subunit

108

3790441

12

0.001

13

61.45/5.23

57.09/5.12

CaM-421¶

Chaperonin 60 alpha^f chain precursor,

78

1710807

4

−0.025

7

61.99/5.15

85.92/6.28

Protein synthesis and degradation

CaM-39*

Putative 60S ribosomal protein L1

101

84468414

3

−0.445

7

44.93/10.40

51.00/4.29

CaM-240*

40S ribosomal protein S12

54

255626071

2

0.106

12

15.34/5.50

19.26/5.37

CaM-283*

40S ribosomal protein S12

162

255626071

3

0.106

23

15.345/5.50

19.02/5.607

CaM-223!

Putative glutamate-tRNA ligase

70

115443869

14

−0.304

11

63.10/6.27

74.10/5.23

CaM-208*

Putative 60S acidic ribosomal protein P0

89

84468360

2

0.031

7

34.31/5.27

40.24/5.10

CaM-209*

Putative 60S acidic ribosomal protein P0

82

84468360

2

0.031

7

34.31/5.27

40.25/5.21

CaM-301*

Proteasome subunit alpha type

199

224139394

4

−0.227

21

27.38/5.59

31.39/5.81

CaM-249!

Polyubiquitin

64

18421671

10

−0.281

27

36.40/5.83

28.56/5.47

CaM-407!

Mitochondrial processing peptidase beta subunit

394

12802327

20

−0.302

22

58.90/6.56

70.46/6.03

Bioenergy

CaM-242*

Cytochrome b6-f complex iron-sulfur subunit

76

136707

2

−0.039

15

16.85/6.45

22.19/5.53

CaM-21!

Chlorophyll a/b-binding^g protein AB96

224

115773

9

0.011

22

24.36/4.98

29.51/4.58

CaM-75*

Chlorophyll a/b binding^g protein

411

3928140

12

−0.068

30

28.35/5.47

30.58/4.78

CaM-196*

Oxygen-evolving enhancer protein 1^h

177

131384

6

−0.303

15

35.44/5.19

35.44/5.19

CaM-294*

Putative PSII-P protein

104

217072770

2

−0.273

8

28.41/7.12

25.41/5.59

CaM-22!

Chlorophyll a/b-binding protein 3 precursor^g

160

115800

7

0.005

19

27.90/5.46

29.38/4.47

CaM-69!

Chlorophyll A-B binding protein^g

118

169124051

8

0.071

12

28.59/5.44

28.05/4.62

CaM-126!

Chlorophyll a/b-binding protein type III (Fragment)^g

65

7271947

4

−0.013

9

20.81/5.17

26.40/4.95

CaM-127!

PSI type III chlorophyll a/b-binding protein (imported)

99

15219941

7

−0.014

11

29.16/8.61

27.98/4.98

CaM-125!

Oxygen-evolving enhancer protein 1^h

111

131384

7

−0.303

14

35.10/6.25

27.83/4.87

CaM-183!

Oxygen-evolving enhancer protein 2

89

131390

9

−0.312

14

28.20/8.29

25.64/5.16

CaM-376*

Ferredoxin NADP reductase

81

125553745

2

−0.340

5

39.95/8.72

36.9/6.12

CaM-184¶

LHCA3, chlorophyll binding

58

79320443

4

−0.009

18

23.84/5.62

28.07/5.23

CaM-74!

Light-harvesting chlorophyll-a/b binding protein Lhcb1

387

56809379

13

−0.083

19

28.43/5.48

30.58/4.93

CaM-319!

Putative ferredoxin-NADP(H) oxidoreductase

67

41052915

7

−0.383

19

41.09/7.98

39.76/5.92

CaM-452¶

Ferredoxin: NADP + reductase

130

4930119

3

−0.403

12

34.98/6.54

36.58/6.49

CaM-226!

ATPase beta subunit (Fragment)ⁱ

276

6467935

18

−0.026

27

51.53/5.22

65.89/5.21

CaM-147*

ATP synthase subunit beta, chloroplasticⁱ

92

11466372

2

0.007

6

51.99/5.10

44.87/4.84

CaM-189*

ATP synthase subunit alpha^j

589

197294119

18

−0.057

24

55.64/5.44

32.38/5.16

CaM-221!

ATP synthase beta subunit (Fragment)ⁱ

116

3850926

13

0.001

22

52.73/5.15

65.80/5.14

CaM-161!

ATP synthase beta subunit (Fragment)ⁱ

918

7708546

41

0.004

51

51.18/5.07

65.76/5.04

CaM-80!

ATP synthase beta subunit (Fragment)ⁱ

671

4063542

19

0.009

26

49.13/5.06

35.75/4.60

CaM-316*

ATP synthase subunit alpha^j

328

197294119

10

−0.057

17

55.64/5.44

45.62/5.83

CaM-222*

ATP synthase subunit betaⁱ

410

197294097

19

−0.016

27

52.96/5.16

73.99/5.16

CaM-335*

ATP synthase subunit alpha^j

75

197294119

2

−0.057

4

55.64/5.44

69.22/5.62

CaM-385¶

ATP synthase subunit gamma, chloroplast precursor

77

226533016

1

−0.143

3

40.10/8.44

41.00/6.06

CaM-311*

ATP synthase CF1 alpha subunit^j

295

289066833

5

−0.045

11

55.73/5.21

42.49/5.89

CaM-396*

ATP synthase subunit alpha^j

439

197294119

10

−0.057

19

55.64/5.44

52.90/5.91

CaM-272!

ATP synthase CF1 alpha subunit^j

572

13518443

21

−0.055

23

55.80/5.22

69.51/5.51

CaM-271!

H + −transporting two-sector ATPase alpha chain

83

114522

13

−0.044

21

54.64/5.75

69.09/5.38

Metabolism

CaM-218*

Glutamine synthetase

50

12963877

1

−0.393

2

47.40/5.73

50.72/5.32

CaM-308*

Fructose-bisphosphate aldolase 1^k

182

399024

5

−0.172

14

38.63/5.83

39.99/5.62

CaM-310¶

Fructose-bisphosphate aldolase (EC 4.1. 2.13) precursor^k

206

399024

6

−0.172

17

38.74/5.83

39.89/5.86

CaM-453*

Fructose-bisphosphate aldolase^k

122

3913008

4

−0.148

14

38.42/6.21

37.10/6.70

CaM-322*

Phosphoglycerate kinase precursor like

124

82621134

5

0.128

11

50.35/8.19

46.94/5.64

CaM-257¶

Plastidic aldolase^k

240

38096041

9

−0.151

17

43.18/6.86

35.52/5.61

CaM-210*

Fructose-bisphosphate aldolase^k

163

84468290

5

−0.140

14

43.03/6.86

40.35/5.35

CaM-460!

Glyceraldehyde 3-phosphate dehydrogenase^l

70

3413165

4

0.069

15

20.88/5.24

41.91/6.66

CaM-216¶

Glutamate-ammonia ligase (EC 6.3.1.2) delta precursor

39

121344

1

−0.390

2

47.65/6.18

51.86/5.13

CaM-115!

Trypsin inhibitor, chain B (fragments)

144

3318877

7

−0.281

57

19.29/4.79

14.86/5.07

CaM-214¶

Glutamate-ammonia ligase (EC 6.3.1.2) delta precursor

68

121344

1

−0.390

2

47.65/6.18

51.14/5.23

CaM-366*

Carbonic anhydrase^m

55

20502881

2

−0.159

4

35.28/6.96

31.01/5.95

CaM-370*

Carbonic anhydrase^m

67

20502881

2

−0.159

5

35.28/6.96

30.85/6.23

CaM-206!

Phosphoribulokinase

71

1885326

6

−0.289

7

39.23/5.41

43.82/5.18

CaM-435*

Carbonic anhydrase^m

95

8954289

3

−0.091

6

35.46/7.59

29.73/6.47

CaM-438*

Carbonic anhydrase^m

56

8954289

2

−0.091

4

35.46/7.59

29.78/6.74

CaM-462*

Glyceraldehyde-3-phosphate dehydrogenase A^l

200

120658

6

−0.047

12

43.31/8.80

44.49/6.81

Miscellaneous

CaM-155!

Dynein-1-alpha heavy chain

69

159490411

63

−0.257

10

525.42/5.32

61.98/5.11

CaM-54!

Activator of spomin

65

145357734

8

−0.634

24

17.25/9.63

12.38/4.69

CaM-289¶

Ribulose-1,5-biphosphate carboxylase/oxygenase large subunit

129

10764577

5

−0.254

10

51.58/5.96

23.69/5.59

CaM-299!

Histone acetyltransferase GCN5

79

18410098

15

−0.581

26

63.48/6.01

30.69/5.63

CaM-38*

Ribulose bisphosphate carboxylase/Oxygenase large chain

60

1750362

2

−0.249

4

52.22/6.09

56.17/4.30

CaM-138*

Chromosome chr17 scaffold_16

149

225456471

4

−0.342

4

33.35/5.67

37.90/4.97

CaM-43*

Ribulose bisphosphate carboxylase large chain

295

197294096

8

−0.277

16

52.65/6.04

64.35/4.29

CaM-290!

Intronic ORF, similar to LAGLIDADG endonuclease

80

2943730

11

−0.486

21

29.46/10.01

21.47/6.00

Unknown function

CaM-443*

Predicted protein

53

224119706

1

0.038

3

27.44/8.65

31.20/6.64

CaM-263¶

OSJNBb0085H11.1 protein

50

38346013

1

-.293

7

176.76/9.26

53.68/5.61

CaM-478*

Putative uncharacterized protein

64

147828109

19

−0.788

0

58.79/6.46

87.91/6.46

CaM-323*

Putative uncharacterized protein

94

224284512

2

−0.395

7

40.97/6.04

44.21/5.66

CaM-477!

Unknown protein

67

27497205

16

−1.250

8

16.57/10.81

65.11/6.62

CaM-171!

Unknown protein

67

15236812

16

−0.827

8

90.90/5.09

74.93/5.11

CaM-64!

Hypothetical protein P0415D04.53

66

47496995

8

−0.587

34

30.31/11.81

24.12/4.77

CaM-267!

Hypothetical protein F26P21.180

67

3688187

10

−0.645

22

57.25/7.64

44.54/5.58

CaM-56!

Hypothetical protein OSJNBa0075N02.148

66

28972006

7

−1.039

29

20.62/12.24

22.37/4.49