The expression pattern of PDF1.2a and PR1 mRNA in Arabidopsis by inoculation of F. sporotrichioides. (A) The expression analysis of PR1 and PDF1.2a mRNA in Arabidopsis after inoculation. ACT2 and ACT8 were used as reference genes. These experiments were repeated 3 times. (B) Activity of MPK3 and MPK6 in response to inoculation with F. sporotrichioides. Upper panel: results of an in-gel kinase assay. Lower panel: CBB staining of the loading control. The amplification efficiency for qRT-PCR of PR-1, PDF1.2 and ACT2, 8 were 91.6, 86.7 and 98.2%, respectively. These experiments were repeated 3 times.