Phosphoproteomic analysis reveals major default phosphorylation sites outside long intrinsically disordered regions of Arabidopsis plasma membrane proteins

Nespoulous, Claude; Rofidal, Valérie; Sommerer, Nicolas; Hem, Sonia; Rossignol, Michel

doi:10.1186/1477-5956-10-62

Proteome Science

Table 2 Identified phosphorylated proteins, peptides and novel sites

From: Phosphoproteomic analysis reveals major default phosphorylation sites outside long intrinsically disordered regions of Arabidopsis plasma membrane proteins

Protein				Peptide				Site
Accession	Protein name	PM SUBA	TMD	Mascot score	PTM score	Location	Sequence	Location	IDR
Transporter
AT1G23080.1	Auxin efflux carrier family protein	PM	10	71	114	[177–201]	VE[S]DVV[S]LDGHDFLETDAQIGDDGK	S179	out
								S183	out
AT1G47670.1	transmembrane amino acid transporter family protein	PM	11	58	163	[18–30]	V[S][T]PEIL[T]PSGQR	S19	in
								T20	in
								T25	in
AT1G48370.1	YELLOW STRIPE like 8	PM	15	58	80	[50–63]	EEQEE[S]VEGIFESR	S55	in
AT1G76430.1	phosphate transporter 1;9		12	49	67	[506–529]	SLEENEDEIVSt(s)AG(s)[S]PANELLR	S522	out
AT2G01420.1	Auxin efflux carrier family protein	PM	10	71	73	[177–201]	VE[S]DVV[S]LDGHDFLETDAEIGNDGK	S179	out
								S183	out
AT2G01980.1	sodium proton exchanger, putative (NHX7) (SOS1)	PM	11	66	90	[1114–1133]	QNTMVE[S][S]DEEDEDEGIVVR	S1120	out
								S1121	out
AT2G28070.1	ABC-2 type transporter family protein	PM	6	54	66	[41–60]	QPISFED[S]PEWED[T]PDVDLR	S48	out
								T54	out
AT2G28120.1	Major facilitator superfamily protein	PM	11	46	102	[556–569]	E[S]PESESELVPDSR	S557	out
AT2G32830.1	phosphate transporter 1;5	PM	12	48	107	[518–538]	EDEEQSGGD[T]VVEMTVANSGR	T527	in
AT3G55320.1	putative subfamily B ABC-type transporter (AtMDR14)	PM	12	46	92	[767–785]	(s)NG(s)EPE[S]PVSPLLTSDPK	S774	in
AT4G23700.1	cation/H+ exchanger 17	PM	12	106	379	[806–820]	NVTTEESLVEDSE[S]P	S819	out
				96	244	[806–820]	NVTTEESLVED[S]E[S]P	S817	out
AT4G24120.1	YELLOW STRIPE like 1	PM	14	98	148	[12–38]	EGEEEEDNNQLSLQEEEPD[T]EEEMSGR	T31	in
AT4G29900.1	autoinhibited Ca(2+)-ATPase 10	PM	9	84	132	[15–37]	DVEAGTS[S]FTEYEDSPFDIASTK	S22	out
AT5G01240.1	like AUXIN RESISTANT 1	PM	10	66	88	[6–22]	QAEESIVV[S]GEDEVAGR	S14	in
				125	202	[24–44]	VED[S]AAEEDIDGNGGNGFSMK	S27	in
AT5G24030.1	SLAC1 homologue 3	PM	9	83	125	[599–610]	NV[S]SENIENYLK	S601	in
AT5G43350.1	phosphate transporter 1;1	PM	12	72	158	[260–270]	VLQ[T]DIELEER	T263	out
				111	70	[509–524]	SLEELSGEAEV[S]HDEK	S520	out
				50	47	[509–524]	[S]LEELSGEAEV[S]HDEK	S509	out
AT5G45380.1	sodium symporters;urea transmembrane transporters	PM	15	86	191	[552–571]	VVEA[Y]ASGDEDVDVPAEELR	Y556	out
AT5G64410.1	oligopeptide transporter 4	PM	15	81	136	[2–19]	ATADEF[S]DEDTSPIEEVR	S8	out
AT5G64560.1	magnesium transporter 9		2	54	53	[130–151]	EIAGAQNDGD[T]GDEDESPFEFR	T140	out
Signalling
AT1G05150.1	Calcium-binding tetratricopeptide family protein	PM		65	65	[171–188]	ADNNNNNVDAFSDAGW[S]R	S187	in
AT1G11330.1	S-locus lectin protein kinase family protein	PM	2	66	57	[556–572]	[S]GQGLEELMNEVVVISK	S556	out
AT1G53440.1	Leucine-rich repeat transmembrane protein kinase	PM	1	56	199	[1025–1035]	LLDDL[T]DVEIE	T1030	in
AT1G55610.1	BRI1 like	PM	1	90	153	[1139–1153]	AD[T]EEDESLDEFSLK	T1141	out
				46	118	[1139–1153]	AD[T]EEDE[S]LDEFSLK	S1146	out
AT1G71860.1	protein tyrosine phosphatase 1	PM		47	106	[19–30]	FDLSSAD[S]PPSK	S26	out
AT3G13380.1	BRI1-like 3	PM	1	87	199	[1134–1151]	ELVQVDTEND[S]LDEFLLK	S1144	out
AT3G13530.1	mitogen-activated protein kinase kinase kinase 7	PM		55	109	[481–510]	VSEGKPNEASTSMPTSNVNQGD[S]PVADGGK	S503	in
AT3G24660.1	Transmembrane kinase-like	PM	2	54	109	[329–350]	K(s)(s)IE[S]EDDLEEGDEEDEIGEK	S334	in
AT3G25070.1	RPM1 interacting protein 4	PM		59	30	[37–61]	IMNPNDPE[Y]NSDSQSQAPPHPPSSR	Y45	in
AT3G28450.1	Leucine-rich repeat protein kinase family protein	PM	2	69	128	[263–276]	[S]GLTEVGVSGLAQR	S263	out
AT3G51740.1	inflorescence meristem receptor-like kinase 2	PM	1	65	155	[755–768]	EEW[T]NEVFDLELMR	T758	out
AT4G23190.1	cysteine-rich RLK (RECEPTOR-like protein kinase) 11	PM	1	61	140	[324–341]	[T]ESE[S]DI[S]TTDSLVYDFK	T324	out
								S328	out
								S331	out
AT4G24630.1	DHHC-type zinc finger family protein	PM	4	58	133	[329–344]	VEDDLDIGDDLMNL[S]R	S343	out
AT4G36180.1	Leucine-rich receptor-like protein kinase family protein	PM	1	74	236	[1119–1136]	VGPDVPSSADPTSQP[S]PA	S1134	out
AT5G05160.1	Leucine-rich repeat protein kinase family protein	PM	1	59	167	[564–577]	EEW[T]AEVFDVELLK	T567	out
AT5G19450.1	calcium-dependent protein kinase 19	PM	1	52	56	[23–41]	SNPFYSEA[Y]TTNGSGTGFK	Y31	in
AT5G58300.1	Leucine-rich repeat protein kinase family protein	PM	1	46	48	[359–366]	A[S]AEVLGK	S360	out
				71	119	[577–590]	EEW[T]SEVFDIELMR	T580	out
AT5G56890.1	Protein kinase superfamily protein	PM	1	77	122	[1041–1055]	YPLLPNYD[S]EPDTER	S1049	in
Miscellaneous
AT1G10340.2	Ankyrin repeat family protein	PM	4	72	31	[331–358]	FGTETSQELD[S]ENNVEQHEGSQEVEVIR	S341	out
AT1G68720.1	tRNA arginine adenosine deaminase	PM		72	119	[1091–1101]	D[S]FEEWEEAYK	S1092	out
AT2G41705.1	camphor resistance CrcB family protein	PM	9	60	111	[31–54]	SLPHLIDNDVD[S]ESVSEAGDIGDR	S42	out
				65	98	[31–54]	SLPHLIDNDVD(s)E[S]V(s)EAGDIGDR	S44	out
				57	101	[31–54]	SLPHLIDNDVD[S]E[S]V[S]EAGDIGDR	S46	out
				60	145	[31–54]	[S]LPHLIDNDVD[S]E[S]V[S]EAGDIGDR	S31	out
				81	112	[68–83]	L[S]ADDFIEQGTHDTSR	S69	in
				63	118	[103–116]	TLPEDITA[S]PLPTK	S111	out
				55	100	[117–128]	SLL[S]PEINNSGK	S120	out
AT3G09770.1	RING/U-box superfamily protein	PM		68	67	[290–317]	YELQEIYGIGN[T]VEGDDDSADDANDPGK	T301	out
AT3G27530.1	golgin candidate 6			74	271	[897–914]	LLEDIGDESEAQAE[S]EED	S911	out
AT3G28850.1	Glutaredoxin family protein	PM		48	111	[18–27]	GY[S]PPVDVQR	S20	out
				56	88	[167–178]	[S]FSFDVGPNGGK	S167	out
				51	18	[381–405]	VYYEYEDDDDDDDEGDDDE[S]VKEER	S400	in
AT4G18950.1	Integrin-linked protein kinase family			84	106	[23–46]	IPEPSVH[S]EEEVFEDGEEIDGGVR	S30	in
AT4G22670.1	HSP70-interacting protein 1			55	69	[57–76]	SFVVEE[S]DDDMDETEEVKPK	S63	in
AT5G07350.1	TUDOR-SN protein 1	PM		51	105	[965–984]	IGIWQYGDIE[S]DDEDTGPAR	S975	out
AT5G44030.1	cellulose synthase A4	PM	8	68	48	[81–100]	IAGDEENNGPDD[S]DDELNIK	S93	in
AT5G49720.1	glycosyl hydrolase 9A1	PM	1	55	152	[5–24]	DPWGGPLEINTAD[S]A[T]DDDR	S18	in
								T20	in
AT5G62390.1	BCL-2-associated athanogene 7	PM		87	321	[429–446]	EIAEGVTQIVQMLE[T]EEE	T443	out
Unknown
AT1G45688.1	unknown protein	PM	1	64	190	[5–19]	TDSEVTSLAAS[S]PAR	S16	out
AT3G27390.1	unknown protein	PM	5	55	219	[536–553]	DNN[S]AKDESITEPPAPVK	S539	out
AT5G64090.1	protein of unknown function	PM	1	50	131	[316–325]	[S]LEIEEDFDR	S316	out

PM SUBA, PM protein location according to the SUBA database; TMD, number of predicted trans-membrane domains according to the Aramemnon database; Mascot score, individual peptide Mascot; PTM score, post-translational modification score calculated using PhosCalc; in/out, inside/outside long IDR according to IUPforest-L predictor. Only peptides containing at least one novel and unambiguous phosphorylated residue are listed (square/normal brackets, unambiguous/ambiguous location according to the PTM score; see Materials and Methods).

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ISSN: 1477-5956

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