Validation of differentially displayed proteins between the neonatal and adult thymus . (A) 60 μg of protein extracts was used for protein detection. There were more abundant ACTN4, CH60 and ACTB expression, while less COF1 expression in neonates. GADPH was used as a normalization control. (B) A summary of densitometric analysis of Western blotting was shown as mean±SE calculated from three experiments. The results were normalized to individual GAPDH expression.