Figure 4From: Label-free quantitative proteomics of CD133-positive liver cancer stem cells Validation of significantly up-regulated proteins in CD133+ Huh7 and CD133- Huh7 cells. (A) RT-PCR, (B) western blot analysis, (C) immunofluorescent staining, and (D) FACS analysis were performed to validate significantly up-regulated proteins in CD133+ and CD133- Huh7 cells. GAPDH in the RT-PCR and β-actin in the western blot were used as loading controls.Back to article page